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First report of plant fungal pathogen Zasmidium musae associated with moribund eggs of ornate spiny lobster (Panulirus ornatus) in Sabah
Aquaculture Reports ( IF 3.7 ) Pub Date : 2020-10-21 , DOI: 10.1016/j.aqrep.2020.100500
Sin Yin Yap , Katsuyuki Hamasaki , Balu Alagar Venmathi Maran , Audrey Daning Tuzan , Choon Looi Ch’ng , Tamrin M. Lal

Fungal infections have been reported as one of the main hindrances for successful crustacean seed production. In a shrimp hatchery of Universiti Malaysia Sabah, abnormal sponge development of the ornate spiny lobster Panulirus ornatus was observed and it was suspected as fungal infection due to a change in sponge colour. Fungus was isolated from egg samples of P. ornatus and transferred to Peptone-Yeast-Glucose-Seawater (PYGS) agar to identify and to reveal its morphological characteristics. Interestingly, the isolate in PYGS broth transferred into sterilized seawater did not show any characteristic feature of any asexual reproduction of Oomycete infection on crustacean. Consequently, slide culture was performed for a more detailed examination, where the fungus isolate showed septate hyphae and vesicle-like fruiting body only upon staining via Iodine-Glycerol stain. Based on the morphological characteristic features, the present isolate was determined as a non-oomycete organism and designated as IPMB LE01 strain. According to the ITS nucleotide sequence analyses of IPMB LE01, it has matched to Zasmidium musae. This species was mainly reported in plant fungal infection and rarely observed in aquatic animals. Hence, it is considered as the first report of fungus-associated on the moribund eggs of ornate spiny lobster in Sabah.



中文翻译:

关于沙巴华丽多刺龙虾(Panulirus ornatus)濒死卵的植物真菌病原体Zasmidium musae的首次报道

据报道,真菌感染是成功生产甲壳类种子的主要障碍之一。在马来西亚沙巴大学的一个虾孵化场中,观察到华丽的多刺龙虾Panulirus ornatus的海绵发育异常,并且由于海绵颜色的变化被怀疑是真菌感染。从雪松P. ornatus的鸡蛋样品中分离出真菌并转移至蛋白-酵母-葡萄糖-海水(PYGS)琼脂中,以鉴定并揭示其形态特征。有趣的是,转移到灭菌海水中的PYGS肉汤中的分离物未显示甲壳类动物的卵菌感染无性繁殖的任何特征。因此,进行了玻片培养以进行更详细的检查,其中真菌分离物仅在通过碘-甘油染色后才显示出隔膜的菌丝和囊样的子实体。基于形态特征,将本分离物确定为非卵菌生物并命名为IPMB LE01菌株。根据IPMB LE01的ITS核苷酸序列分析,它与百日草匹配。该物种主要报道在植物真菌感染中,很少在水生动物中观察到。因此,它被认为是沙巴华丽多刺龙虾垂死卵上真菌相关的首次报道。

更新日期:2020-10-30
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