Abstract

Phase transformations of bovine serum albumin (BSA) are investigated using the Brilluoin light scattering in the temperature range from 300 to 380 K in solutions with concentrations of 50 and 5 mg/mL. As the reference sequence, the sequence of phase transformations in a concentrated (100 mg/mL) BSA solution are used for analyzing the results. It is shown that the sequence of BSA phase transformations is modified upon a decrease in the concentration. For example, in the vicinity of denaturation, the region with fibrilform protein aggregates disappears, and the properties of gel-type high-temperature phase change.

中文翻译：

---

在热变性附近浓度不同的牛血清白蛋白溶液中的Mandelstam–Brillouin光散射

摘要

牛血清白蛋白（BSA）的相变是在浓度为50和5 mg / mL的溶液中，在300至380 K的温度范围内使用布里渊光散射进行研究的。作为参考序列，将浓（100 mg / mL）BSA溶液中的相变序列用于分析结果。结果表明，随着浓度的降低，BSA相变的顺序被改变。例如，在变性附近，具有纤维状蛋白聚集体的区域消失，并且凝胶型高温相的性质改变。