Abstract

Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous and complex disease, both from a clinical and molecular point of view. The prolonged use of alcohol and tobacco, along with the release of tumor secretions can modulate blood cells, such as erythrocytes. Here, this study was conducted with 24 patients diagnosed with HNSCC and an equal number of healthy individuals are matched by age and gender. The levels of lipid peroxidation were measured using the individual plasma, while for lipid concentrations, identification and quantification Na, K-ATPase activity and osmotic fragility, the red blood cell concentrate were used. The release of TBARS was significantly higher in patients with HNSCC. The lipid profile assays demonstrated a rearrangement of the erythrocyte membrane due to a decrease in total phospholipids and phosphatidylethanolamine followed by an increase in total cholesterol and phosphatidylcholine. Na, K-ATPase activity also increased. Erythrocytes were more fragile in patients with HNSCC than in health individuals. Therefore, the membrane of erythrocytes were rearranged and Na, K-ATPase function altered in the HNSCC patients. Our findings suggests that the alcohol, tobacco and tumor secretion modulate in a specific manner that the erythrocytes membranes of these patients making this system a potential tool for HNSCC biomarker of tumor progression.

Graphic Abstract

中文翻译：

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头颈癌患者红细胞膜的评价

摘要

从临床和分子的角度来看，头颈部鳞状细胞癌 (HNSCC) 是一种异质性和复杂的疾病。长期使用酒精和烟草，随着肿瘤分泌物的释放，可以调节血细胞，如红细胞。在这里，这项研究是对 24 名诊断为 HNSCC 的患者进行的，同样数量的健康个体按年龄和性别进行匹配。使用单个血浆测量脂质过氧化水平，而对于脂质浓度、鉴定和定量 Na、K-ATP 酶活性和渗透脆性，使用红细胞浓缩物。HNSCC 患者中 TBARS 的释放显着更高。脂质谱分析表明，由于总磷脂和磷脂酰乙醇胺减少，随后总胆固醇和磷脂酰胆碱增加，红细胞膜发生重排。Na、K-ATPase 活性也增加。HNSCC 患者的红细胞比健康人更脆弱。因此，HNSCC 患者的红细胞膜发生重排，Na、K-ATPase 功能发生改变。我们的研究结果表明，酒精、烟草和肿瘤分泌以特定方式调节这些患者的红细胞膜，使该系统成为 HNSCC 肿瘤进展生物标志物的潜在工具。HNSCC 患者的红细胞比健康人更脆弱。因此，HNSCC 患者的红细胞膜发生重排，Na、K-ATPase 功能发生改变。我们的研究结果表明，酒精、烟草和肿瘤分泌以特定方式调节这些患者的红细胞膜，使该系统成为 HNSCC 肿瘤进展生物标志物的潜在工具。HNSCC 患者的红细胞比健康人更脆弱。因此，HNSCC 患者的红细胞膜发生重排，Na、K-ATPase 功能发生改变。我们的研究结果表明，酒精、烟草和肿瘤分泌以特定方式调节这些患者的红细胞膜，使该系统成为 HNSCC 肿瘤进展生物标志物的潜在工具。

图形摘要