Key message

The promoter of the Arabidopsis thaliana β-glucosidase 19 gene directs GUS expression in a seed-specific manner in transgenic Arabidopsis and tobacco.

Abstract

In the present study, an 898-bp putative promoter of the Arabidopsis β-glucosidase 19 (AtBGLU19) gene was cloned. The bioinformatics analysis of the cis-acting elements indicated that this putative promoter contains many seed-specific elements, such as RY elements. The features of this promoter fragment were evaluated for the capacity to direct the β-glucuronidase (GUS) reporter gene in transgenic Arabidopsis and tobacco. Histochemical and fluorometric GUS analyses of transgenic Arabidopsis plants revealed that the AtBGLU19 promoter directed strong GUS activity in late-maturing seeds and dry seeds, whereas no GUS expression was observed in other organs. The results indicated that the AtBGLU19 promoter was able to direct GUS expression in a seed-specific manner in transgenic Arabidopsis. In tobacco, the intensity of the staining and the level of GUS activity were considerably higher in the seeds than in the other tissues. These results further confirmed that the AtBGLU19 promoter is seed specific and can be used to control transgene expression in a heterologous plant system.

中文翻译：

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拟南芥β-葡萄糖苷酶19启动子在转基因拟南芥和烟草中的种子特异性活性

关键信息

拟南芥β-葡萄糖苷酶 19 基因的启动子在转基因拟南芥和烟草中以种子特异性方式指导 GUS 表达。

抽象的

在本研究中，克隆了 拟南芥β-葡萄糖苷酶 19 ( AtBGLU19 ) 基因的 898-bp 推定启动子。顺式作用元件的生物信息学分析表明，这种推定的启动子包含许多种子特异性元件，例如 RY 元件。评估了该启动子片段的特征，以确定在转基因拟南芥和烟草中引导 β-葡萄糖醛酸酶 ( GUS ) 报告基因的能力。转基因拟南芥植物的组织化学和荧光 GUS 分析表明AtBGLU19启动子在晚熟种子和干燥种子中引导强 GUS 活性，而在其他器官中未观察到 GUS 表达。结果表明，AtBGLU19启动子能够以种子特异性方式在转基因拟南芥中指导 GUS 表达。在烟草中，种子中的染色强度和 GUS 活性水平明显高于其他组织。这些结果进一步证实AtBGLU19启动子具有种子特异性，可用于控制异源植物系统中的转基因表达。