EST-SSR markers were developed from Pongamia pinnata transcriptome libraries. We have successfully utilised EST-SSRs to study the genetic diversity of Indian P. pinnata germplasms and transferability study on legume plants. P. pinnata is a non-edible oil, seed-bearing leguminous tree well known for its multipurpose benefits and acts as a potential source for medicine and biodiesel preparation. Moreover, the plant is not grazable by animal and wildly grown in different agro climatic condition of India. Recently, it is much used in reforestation and rehabilitation of marginal and coal mined land in different part of India. Due to increasing demand for cultivation, understanding of the genetic diversity is important parameter for further breeding and cultivation program. In this investigation, an attempt has been undertaken to develop novel EST-SSR markers by analyzing the assembled transcriptome from previously published Illumina libraries of P. pinnata, which is cross transferrable to legume plants. Twenty EST-SSR markers were developed from oil yielding and secondary metabolite biosynthesis genes. To our knowledge, this is the first EST-SSR marker based genetic diversity study on Indian P. pinnata germplasms. The genetic diversity parameter analysis of P. pinnata showed that the Gangetic plain and Eastern India are highly diverse compared to the Central Deccan and Western germplasms. The lowest genetic diversity in the Western region may be due to the pressure of lower precipitation, high-temperature stress and reduced groundwater availability. Nevertheless, the highest genetic diversity of Gangetic plain and Eastern India may be due to the higher groundwater availability, high precipitation, higher temperature fluctuations and growing by the side of glacier-fed river water. Thus, our study shows the evidence of natural selection on the genetic diversity of P. pinnata germplasms of the Indian subcontinent.

EST-SSR 标记是从Pongamia pinnata转录组文库中开发的。我们成功地利用EST-SSRs研究了印度羽衣甘蓝种质的遗传多样性和豆科植物的可转移性研究。羽扇豆是一种非食用油、含种子的豆科树，以其多用途的益处而闻名，是医药和生物柴油制备的潜在来源。此外，该植物不可被动物放牧，而是在印度不同的农业气候条件下广泛种植。最近，它被广泛用于印度不同地区的边缘和煤矿土地的重新造林和恢复。由于对栽培的需求不断增加，了解遗传多样性是进一步育种和栽培计划的重要参数。在这项调查中，通过分析来自先前发表的 Illumina 的P. pinnata文库的组装转录组，已尝试开发新的 EST-SSR 标记，可交叉转移到豆科植物。20 个 EST-SSR 标记是从产油基因和次生代谢物生物合成基因中开发出来的。据我们所知，这是第一个基于 EST-SSR 标记的印度羽扇豆种质遗传多样性研究。羽扇豆遗传多样性参数分析表明与中德干和西部种质相比，恒河平原和印度东部具有高度多样性。西部地区遗传多样性最低的原因可能是降水减少、高温压力和地下水可用量减少的压力。然而，恒河平原和印度东部的最高遗传多样性可能是由于地下水可用量较高、降水量大、温度波动较大以及在冰川河水边生长。因此，我们的研究显示了自然选择对印度次大陆P. pinnata种质的遗传多样性的证据。