Transdermal delivery of drugs represents a non-invasive alternative treatment used not only for skin diseases. As one of the possible penetration enhancing agents, various types of nanoparticles (NPs) could be used. Silver NPs (AgNPs) could be used for some medical purposes considering their antibacterial and antiinflammatory properties. We demonstrate a novel method of quantification of permeated AgNPs, detection of AgNPs dissolving while passing the skin, and examination of interactions between skin and systems with AgNPs. Several AgNPs (exhibiting defined mean diameters of 20, 40, 60, and 100 nm) were added individually to the pure solvents commonly used in pharmaceuticals, namely ethanol, methanol, dimethyl sulfoxide, and demineralized water. AgNP dispersions in different solvents were applied to untreated samples of the skin. Attenuated total reflection technique was used for monitoring the kinetic series of infrared spectra to elucidate the time-dependent changes in the uppermost layer of the skin. The depth profiling spectra series were measured using confocal Raman microspectrometer. All recorded vibrational spectra were evaluated by multivariate statistical methods. A strong influence of AgNP size on the structural changes of the skin surface was evident. The largest changes of the skin structure were caused by the 20-nm and 40-nm AgNPs. Permeation of used AgNPs was studied on vertical Franz diffusion cells with detection of permeated AgNPs by new method based on single-particle inductively coupled plasma mass spectrometry.

药物的透皮递送代表了不仅用于皮肤疾病的非侵入性替代治疗。作为可能的渗透促进剂之一，可以使用各种类型的纳米颗粒（NP）。考虑到它们的抗菌和消炎特性，它们可以用于某些医疗目的。我们演示了一种新方法，可以量化渗透的AgNPs，检测通过皮肤时溶解的AgNPs以及检查皮肤与AgNPs系统之间的相互作用。将几种AgNP（分别具有20、40、60和100 nm的定义平均直径）分别添加到药物常用的纯溶剂中，即乙醇，甲醇，二甲基亚砜和软化水。将AgNP在不同溶剂中的分散液应用于未经处理的皮肤样品。衰减全反射技术用于监测红外光谱的动力学序列，以阐明皮肤最上层随时间的变化。使用共聚焦拉曼光谱仪测量深度分布谱系列。所有记录的振动光谱通过多元统计方法进行评估。很明显，AgNP大小对皮肤表面结构变化有很大影响。皮肤结构的最大变化是由20 nm和40 nm AgNP引起的。研究了用过的AgNPs在垂直Franz扩散池中的渗透，并通过基于单粒子感应耦合等离子体质谱的新方法检测渗透的AgNPs。