Abstract

The effect of noopept (N-phenylacetyl-L-prolyl-glycine ethyl ester) on the DNA-binding activity of HIF-1 in SH-SH5Y cells and the mechanisms of stabilization of this transcription factor were studied in vitro. Noopept was shown to increase both the basal DNA-binding activity of HIF-1 and the activity induced by various hypoxia mimetics. The mechanism of stabilization of the oxygen-sensitive HIF1α subunit by noopept involves the inhibition of HIF-1 prolyl hydroxylase, which is indirectly indicated by the data obtained using the ODD-Luc reporter, and the positive effect on the level of the HIF1α protein. It was revealed that the effect of noopept is accompanied by changes in gene expression, which belong to different metabolic pathways and are controlled by the transcription factor HIF-1.

中文翻译：

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认知增强剂Noeptept激活转录因子HIF-1

摘要

noopept的（效果Ñ -phenylacetyl-大号体外研究了HIF-1在SH-SH5Y细胞中的DNA结合活性以及该转录因子的稳定机制。已显示Noopept可同时增加HIF-1的基础DNA结合活性和各种低氧模拟物诱导的活性。Noeptept对氧敏感的HIF1α亚基的稳定机制包括抑制HIF-1脯氨酰羟化酶（使用ODD-Luc报告基因间接获得的数据间接表明了抑制作用）以及对HIF1α蛋白水平的积极影响。揭示了noopept的作用伴随着基因表达的变化，该变化属于不同的代谢途径，并受转录因子HIF-1的控制。