Neuroblastoma is one of the most widely seen under the age of 15 tumors that occur in the adrenal medulla and sympathetic ganglia. Cisplatin, an antineoplastic drug, is a Platinum-based compound and is known to inhibit the proliferation of neuroblastoma cells. Effective applications of nanoparticles in biomedical areas such as biomolecular, antimicrobial detection and diagnosis, tissue engineering, theranostics, biomarking, drug delivery, and anti-cancer have been investigated in many studies. This study aims to prepare the bioconjugates of CoS (cobalt sulfide) nanoparticles (NPs) with cisplatin combination groups and to evaluate their effects on the neuroblastoma cell line. Nanoparticle synthesis was done using the green synthesis technique using Punica granatum plant extract. The size and shape of CoS NPs were characterized by SEM, FT-IR, and XRD. Zeta potential was confirmed by the DLS study. For this purpose, the SH-SY5Y neuroblastoma cell line was cultured in a suitable cell culture medium. Cisplatin 5 µg and different concentrations (Cisplatin + CoS NPs bioconjugates (5, 10, 25, 50, 75 μg) doses were applied to SH-SY5Y neuroblastoma cell lines for 24 h. TAC, TOS and MTT tests were performed 24 h after the application. According to the MTT test results, cisplatin and CoS NP combinations reduced the proliferation of neuroblastoma cells by 78 to 57% compared to the cisplatin control. From the findings obtained; the most effective Bio-conjugate group was Cisplatin 5 μg/mL + CoS 75 μg/mL.

神经母细胞瘤是发生在肾上腺髓质和交感神经节的 15 岁以下最常见的肿瘤之一。顺铂是一种抗肿瘤药物，是一种基于铂的化合物，已知可抑制神经母细胞瘤细胞的增殖。许多研究已经研究了纳米粒子在生物医学领域的有效应用，例如生物分子、抗菌检测和诊断、组织工程、治疗诊断学、生物标记、药物递送和抗癌。本研究旨在制备 CoS（硫化钴）纳米粒子 (NPs) 与顺铂组合组的生物共轭物，并评估它们对神经母细胞瘤细胞系的影响。使用石榴石的绿色合成技术合成纳米颗粒植物提取物。CoS NPs 的大小和形状通过 SEM、FT-IR 和 XRD 进行表征。DLS 研究证实了 Zeta 电位。为此，将 SH-SY5Y 神经母细胞瘤细胞系在合适的细胞培养基中培养。将顺铂 5 µg 和不同浓度（顺铂 + CoS NPs 生物偶联物（5、10、25、50、75 µg）剂量应用于 SH-SY5Y 神经母细胞瘤细胞系 24 小时。TAC、TOS 和 MTT 测试在注射后 24 小时进行应用。根据 MTT 测试结果，与顺铂对照相比，顺铂和 CoS NP 组合使神经母细胞瘤细胞的增殖降低了 78% 至 57%。从获得的结果来看，最有效的生物偶联物组是顺铂 5 μg/mL + CoS 75 μg/mL。