Only a limited number of techniques are available for assessing the effect of different coating materials on cell adherence to screws. In this study, we describe a simple and inexpensive method for evaluation of cell adhesion on irregular surfaces such as the surgical or implant screws. For this purpose, we prepared semi-submerged screws in the petri dishes using agar. Using BSA- or HA-coated screws, we tested whether BSA or HA could improve cell adherence when used as coating materials. Agar-coated screws were used as internal control. Then the “ratio of cell adherence” was calculated by subtracting the reference RCA value obtained from the agar coated screws (internal control). When compared to that of the non-coated screws both the HA- and BSA-coating improved cell adherence on the screws by 2.34 and 2.72 fold respectively. Similarly, MTT assay data revealed that the metabolic capacities of cells on HA- or BSA-coated screws were improved by 2.36 and 2.86 fold respectively. These findings suggest that this protocol can be used for comparing the ability of cells to attach on irregular surfaces such as dental or orthopedic screws and assessing their viability.

只有有限数量的技术可用于评估不同涂层材料对细胞粘附到螺钉的影响。在这项研究中，我们描述了一种简单且廉价的方法，用于评估不规则表面（如手术或植入螺钉）上的细胞粘附。为此，我们使用琼脂在培养皿中制备了半浸入式螺钉。使用 BSA 或 HA 涂层螺钉，我们测试了 BSA 或 HA 在用作涂层材料时是否可以提高细胞粘附。琼脂包被螺钉用作内部对照。然后通过减去从琼脂包被的螺钉（内部对照）获得的参考 RCA 值来计算“细胞粘附的比率”。与非涂层螺钉相比，HA 涂层和 BSA 涂层将螺钉上的细胞粘附分别提高了 2.34 和 2.72 倍。相似地，MTT 测定数据显示，HA 或 BSA 涂层螺钉上细胞的代谢能力分别提高了 2.36 和 2.86 倍。这些发现表明，该协议可用于比较细胞附着在不规则表面（如牙科或骨科螺钉）上的能力并评估其生存能力。