The infection induced by implantation of biomedical materials may result from the biofilm formation after bacteria attachment. Hence, the antibiofilm surface coating represents a novel technique to improve the antibacterial activity of biomedical materials. The traditional antibiofilm surface coatings exhibited some disadvantages and provided a limited service life. In this work, we used polyethyleneimine grafted 3-maleimidopropionic acid (PEIM) and poly(acrylic acid) grafted 2-furfurylamine (PAAF) to achieve robust and self-healable crosslinked multilayer coatings, employing Layer-by-Layer (LbL) self-assembly technique and Diels-Alder reaction. Then, thiol-terminated poly((3-acrylamidopropyl) trimethylammonium chloride) (PAMPTMA-SH) was grafted onto the crosslinked multilayer coating by thiol-ene click reaction to form a novel multilayer coating (PEIM/PAAF)₁₀-PAMPTMA. We found that this coating showed robust and self-healable activity, and significantly inhibited the bacterial growth and biofilm formation after infection with Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) by in vitro and in vivo assays for 120 h. In addition, the multilayer coating did not induce significant hemolysis or affect the cell viability of red blood cells. In vivo studies also showed that (PEIM/PAAF)₁₀-PAMPTMA coating efficiently blocked the infiltration of inflammatory cells and gene expression in the mouse skin challenged with E. coli or S. aureus. Taken together, these results showed that the prepared multilayer coating exhibited strong antibiofilm activity and provided a new strategy for the application of highly efficient antibiofilm surface coating of biomedical materials.

由生物医学材料的植入引起的感染可能是由于细菌附着后生物膜的形成。因此，抗生物膜表面涂层代表了一种改善生物医学材料抗菌活性的新技术。传统的抗生物膜表面涂层显示出一些缺点并且使用寿命有限。在这项工作中，我们使用了聚乙烯亚胺接枝的3-马来酰亚胺基丙酸（PEIM）和聚（丙烯酸）接枝的2-糠胺（PAAF），从而实现了坚固且可自愈的交联多层涂料，并采用了逐层（LbL）自涂组装技术和Diels-Alder反应。然后，₁₀ -PAMPTMA。我们发现该涂层表现出强大的自我修复活性，并通过体外和体内试验120 h显着抑制了大肠杆菌（E. coli）和金黄色葡萄球菌（S. aureus）感染后细菌的生长和生物膜的形成。。另外，多层涂层没有引起明显的溶血或影响红细胞的细胞活力。体内研究还表明，（PEIM / PAAF）₁₀ -PAMPTMA涂层可有效阻断炎症细胞的浸润和基因表达，使其受到大肠杆菌或大肠杆菌攻击。金黄色葡萄球菌。综上所述，这些结果表明，所制备的多层涂层表现出很强的抗生物膜活性，并为应用生物医学材料的高效抗生物膜表面涂层提供了新的策略。