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3D printed hydrogels with oxidized cellulose nanofibers and silk fibroin for the proliferation of lung epithelial stem cells
Cellulose ( IF 5.7 ) Pub Date : 2020-10-26 , DOI: 10.1007/s10570-020-03526-7
Li Huang 1 , Wei Yuan 2 , Yue Hong 3 , Suna Fan 1 , Xiang Yao 1 , Tao Ren 3 , Lujie Song 4, 5 , Gesheng Yang 1 , Yaopeng Zhang 1
Affiliation  

A novel biomaterial ink consisting of regenerated silk fibroin (SF) and 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)-oxidized bacterial cellulose (OBC) nanofibrils was developed for 3D printing lung tissue scaffold. Silk fibroin backbones were cross-linked using horseradish peroxide/H2O2 to form printed hydrogel scaffolds. OBC with a concentration of 7wt% increased the viscosity of inks during the printing process and further improved the shape fidelity of the scaffolds. Rheological measurements and image analyses were performed to evaluate inks printability and print shape fidelity. Three-dimensional construct with ten layers could be printed with ink of 1SF-2OBC (SF/OBC = 1/2, w/w). The composite hydrogel of 1SF-1OBC (SF/OBC = 1/1, w/w) printed at 25 °C exhibited a significantly improved compressive strength of 267 ± 13 kPa and a compressive stiffness of 325 ± 14 kPa at 30% strain, respectively. The optimized printing parameters for 1SF-1OBC were 0.3 bar of printing pressure, 45 mm/s of printing speed and 410 μm of nozzle diameter. Furthermore, OBC nanofibrils could be induced to align along the print lines over 60% degree of orientation, which were analyzed by SEM and X-ray diffraction. The orientation of OBC nanofibrils along print lines provided physical cues for guiding the orientation of lung epithelial stem cells, which maintained the ability to proliferate and kept epithelial phenotype after 7 days’ culture. The 3D printed SF-OBC scaffolds are promising for applications in lung tissue engineering.



中文翻译:

具有氧化纤维素纳米纤维和丝素蛋白的 3D 打印水凝胶用于肺上皮干细胞的增殖

开发了一种由再生丝素蛋白 (SF) 和 2,2,6,6-四甲基哌啶-1-氧基 (TEMPO)-氧化细菌纤维素 (OBC) 纳米纤维组成的新型生物材料墨水,用于 3D 打印肺组织支架。丝素蛋白骨架使用辣根过氧化物/H 2 O 2交联形成印刷的水凝胶支架。浓度为 7wt% 的 OBC 在印刷过程中增加了油墨的粘度,进一步提高了支架的形状保真度。进行流变测量和图像分析以评估油墨的印刷适性和印刷形状保真度。可以用 1SF-2OBC (SF/OBC = 1/2, w/w) 的墨水打印具有十层的三维结构。在 25 °C 打印的 1SF-1OBC (SF/OBC = 1/1, w/w) 复合水凝胶在 30% 应变下表现出显着提高的 267 ± 13 kPa 的抗压强度和 325 ± 14 kPa 的抗压刚度,分别。1SF-1OBC 的优化打印参数为 0.3 bar 的打印压力、45 mm/s 的打印速度和 410 μm 的喷嘴直径。此外,可以诱导 OBC 纳米纤维在 60% 的取向度上沿打印线排列,通过 SEM 和 X 射线衍射对其进行分析。OBC 纳米纤维沿打印线的方向为指导肺上皮干细胞的方向提供了物理线索,在 7 天的培养后,肺上皮干细胞保持了增殖能力并保持了上皮表型。3D 打印的 SF-OBC 支架有望用于肺组织工程。

更新日期:2020-10-30
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