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Attenuation of morpho-anatomical disorders of micropropagated plantlets of Crinum malabaricum Lekhak and Yadav using seismo-mechanical stimulation
Brazilian Journal of Botany ( IF 1.6 ) Pub Date : 2020-10-27 , DOI: 10.1007/s40415-020-00670-z
S. Priyadharshini , M. Manokari , Mahipal S. Shekhawat

The micropropagation of critically endangered plant Crinum malabaricum Lekhak and Yadav (Malabar river lily) resulted in the development of in vitro-induced structural and physiological aberrations in the plantlets, which hampered the successful acclimatization and field establishment. Therefore, this study aimed to identify the effects of seismic stress on the morpho-anatomical developments in tissue culture raised plantlets of C. malabaricum. The cultures were incubated for 4 weeks at 100 revolutions per min (rpm) for 8 min and twice per day. The mild mechanical agitation in the incubator shaker induced rapid changes in the morphology of stomata, epidermal cells, cuticular wax deposition, mesophyll cells, vascular elements, and development of air cavities in the leaves. It also improved the number of shoots (63.0 ± 0.16), leaf area (12.5 ± 0.24 cm length and 2.2 ± 0.18 cm width), number of roots (12.0 ± 0.20), and fresh weight (3.5 ± 0.29 gm) of plantlets per culture vessel. These parameters were reasonably elevated as compared to the non-stressed plantlets. The seismic stress also enhanced photosynthetic pigments ratio (7.36 ± 0.24 μg g−1 FW) and morpho-anatomical developments in the in vitro plantlets, and ultimately induced tolerance to withstand the stressful conditions of ex vitro and in vivo conditions. The study could help in improvement of the survival success of tissue culture raised plantlets via in vitro acclimatization. Thus, it could serve as a conservation strategy for this critically endangered and medicinally important plant species.

中文翻译:

使用地震机械刺激减弱 Crinum malabaricum Lekhak 和 Yadav 微繁小苗的形态解剖学障碍

极度濒危植物 Crinum malabaricum Lekhak 和 Yadav(马拉巴河百合)的微繁殖导致幼苗体外诱导的结构和生理畸变的发展,这阻碍了成功的驯化和田间建立。因此,本研究旨在确定地震应力对 C. malabaricum 组织培养培养的小植株形态解剖学发展的影响。将培养物以 100 转/分钟 (rpm) 的速度培养 8 分钟,每天培养两次。培养箱振动器中的轻微机械搅拌引起气孔、表皮细胞、表皮蜡沉积、叶肉细胞、维管元素和叶片中气穴发育的形态的快速变化。它还提高了芽数 (63.0 ± 0.16)、叶面积 (12.5 ± 0. 每个培养容器的幼苗长 24 厘米,宽 2.2 ± 0.18 厘米)、根数 (12.0 ± 0.20) 和鲜重 (3.5 ± 0.29 克)。与未受胁迫的小植株相比,这些参数被合理地提高。地震应力还提高了体外幼苗的光合色素比率(7.36 ± 0.24 μg g-1 FW)和形态解剖学发展,并最终诱导耐受体外和体内条件的压力条件。该研究可以帮助通过体外驯化提高组织培养培育的幼苗的成活率。因此,它可以作为这种极度濒危且具有重要药用价值的植物物种的保护策略。与未受胁迫的小植株相比,这些参数被合理地提高。地震应力还提高了体外幼苗的光合色素比率(7.36 ± 0.24 μg g−1 FW)和形态解剖学发展,并最终诱导耐受体外和体内条件的压力条件。该研究可以帮助通过体外驯化提高组织培养培育的幼苗的成活率。因此,它可以作为这种极度濒危且具有重要药用价值的植物物种的保护策略。与未受胁迫的小植株相比,这些参数被合理地提高。地震应力还提高了体外幼苗的光合色素比率(7.36 ± 0.24 μg g-1 FW)和形态解剖学发展,并最终诱导耐受体外和体内条件的压力条件。该研究可以帮助通过体外驯化提高组织培养培育的幼苗的成活率。因此,它可以作为这种极度濒危且具有重要药用价值的植物物种的保护策略。并最终诱导耐受体外和体内条件的压力条件。该研究可以帮助通过体外驯化提高组织培养培育的幼苗的成活率。因此,它可以作为这种极度濒危且具有重要药用价值的植物物种的保护策略。并最终诱导耐受体外和体内条件的压力条件。该研究可以帮助通过体外驯化提高组织培养培育的幼苗的成活率。因此,它可以作为这种极度濒危且具有重要药用价值的植物物种的保护策略。
更新日期:2020-10-27
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