Archives of Virology ( IF 2.7 ) Pub Date : 2020-09-25 , DOI: 10.1007/s00705-020-04814-0 Leonardo A. Silva , Carolina Jordan , Vanessa Rafaela de Carvalho , Carlos F. Wilcken , Bergmann M. Ribeiro
The genomes of two putative new RNA viruses (macula-like virus and bunya-like virus) were identified in total RNA extracted from dead eucalyptus snout beetles (Gonipterus spp.) from a laboratory colony. However, only bunya-like virus was detected in field-collected insects. The macula-like virus has a monopartite single-stranded RNA genome that contains three open reading frames (ORFs) encoding an RNA-dependent RNA polymerase (RdRp), a capsid protein (CP), protein with unknown function. The bunya-like virus genome was predicted to consist of two RNA segments: a large segment (L) encoding a single protein (RdRp) and a small segment (S) encoding a putative nucleocapsid protein.
中文翻译:
桉树鼻子甲虫Gonipterus spp中RNA病毒的鉴定和基因组测序。(鞘翅目:Curculionidae)
在从实验室菌落的死桉树鼻金龟甲虫(Gonipterus spp。)中提取的总RNA中,鉴定出两种推定的新RNA病毒(类黄斑病毒和类布尼亚病毒)的基因组。但是,在田间采集的昆虫中仅检测到类布尼亚病毒。黄斑样病毒具有单部分的单链RNA基因组,其中包含三个开放阅读框(ORF),它们编码RNA依赖性RNA聚合酶(RdRp),衣壳蛋白(CP)和功能未知的蛋白。预测该类布尼亚病毒基因组由两个RNA片段组成:编码单个蛋白质(RdRp)的大片段(L)和编码推定的核衣壳蛋白的小片段(S)。