Culture medium composition is one of the most important parameters to analyze in biotechnological processes with industrial purposes. The aim of this study was to design of a culture medium for optimal growth of the bacterium Pseudoxanthomonas indica H32 allowing its production as biopesticide and biofertilizer. The influence of several carbon and nitrogen sources and their molar ratios on P. indica H32 growth was investigated. The effect of different micronutrients such as mineral salts and vitamin on P. indica H32 growth was determined as well. A mixture design based on Design-Expert 10.0 Software was performed to optimize the culture medium concentration. Finally, in the designed medium, an attribute of the biological mechanism of action of the P. indica H32 against nematodes, was evaluated: the hydrogen sulfide production. It was found that tested carbon/nitrogen ratios were not a significant influence on P. indica H32 growth. Growth of P. indica H32 was favored with use of sucrose, yeast extract and phosphate buffer without the addition of any tested micronutrients. An optimal concentration of 10 g/L sucrose and 5 g/L yeast extract were obtained at a cost of 0.10 $/L. In this concentration, the specific growth rate (µ) and maximal optical density (Xmax) were equal to 0.439 h^− 1 and 8.00 respectively. It was evidenced that under the culture conditions used, P. indica H32 produced hydrogen sulfide. The designed medium led to a 1.08 $/L reduction of costs in comparison to LB medium. These results were critical to carry on with biotechnological development of P. indica H32 as a bioproduct.

培养基成分是在具有工业用途的生物技术过程中进行分析的最重要参数之一。这项研究的目的是设计一种培养基，以使in假单胞菌H32细菌最佳生长，使其能够作为生物农药和生物肥料生产。研究了几种碳源和氮源及其摩尔比对印度PH32生长的影响。还确定了不同的微量营养元素，如矿物质盐和维生素，对P稻H32生长的影响。进行了基于Design-Expert 10.0软件的混合物设计，以优化培养基浓度。最后，在设计的介质中，评价了对线虫的印度.H32：硫化氢的产生。发现所测试的碳/氮比对印度PH32的生长没有显着影响。使用蔗糖，酵母提取物和磷酸盐缓冲液而不添加任何测试的微量营养元素，有利于印度HH32的生长。获得最佳浓度的10 g / L蔗糖和5 g / L酵母提取物的成本为0.10 $ / L。在此浓度下，比生长速率（µ）和最大光密度（Xmax）分别等于0.439 h ^-1和8.00。有证据表明在所用的培养条件下，印度PH32产生硫化氢。与LB培养基相比，设计的培养基导致成本降低1.08 $ / L。这些结果对于继续进行作为生物产品的印度假单胞菌H32的生物技术开发至关重要。