Supplementation of the spent oyster substrate enhances its nutritional properties to produce a new mushroom cropping cycle. The study investigated the potential of a nano-fertilizer (Lithovit^®-Amino25) with an admixture of 25% l-amino acids on Pleurotus ostreatus production, proteins, and amino acid contents. The product applied at spawning (t1), after the first harvest (t2), and at both timings (t3), in two doses: 3 g/kg (C1) or 5 g/kg (C2). Compared with control (C0t0), the first harvest was earlier by 2.3–3.3 days in C1t1 and C2t1. The biological yield of the second harvest was improved by 28.0% in C2t2. Superior results were in C1t3 where the number of crops increased to four, biological efficiency was optimized (117.3%) at the third harvest, and biological and economic yields increased by 36.7% and 36.4%, respectively. Lignin was the most degraded in C1t3, while residual cellulose, hemicellulose, neutral detergent fiber, and acid detergent fiber were higher in all treated substrates than in control. In C2t1, mushrooms were the richest in proteins, while in C1t1, they were the richest in the essential amino acids threonine, valine, isoleucine, leucine, and histidine. Lithovit^®-Amino25 has a high potential for use in P. ostreatus production.

补充废牡蛎基质可增强其营养特性，从而产生新的蘑菇种植周期。该研究调查了纳米肥料（Lithovit的电位^® -Amino25）具有25％的混合物升α-氨基酸上平菇产量，蛋白质和氨基酸含量。该产品在产卵期（t1），第一次收获后（t2）和两个时间点（t3）均以两种剂量施用：3 g / kg（C1）或5 g / kg（C2）。与对照（C0t0）相比，C1t1和C2t1的首次收获提前了2.3-3.3天。在C2t2中，第二次收获的生物产量提高了28.0％。C1t3取得了优异的成绩，其中农作物的数量增加到了4种，第三次收获时的生物效率得到了优化（117.3％），生物和经济产量分别提高了36.7％和36.4％。木质素在C1t3中降解程度最高，而所有处理过的底物中的残留纤维素，半纤维素，中性去污剂纤维和酸性去污剂纤维均高于对照。在C2t1中，蘑菇中的蛋白质含量最高，而在C1t1中，它们是必需氨基酸中最丰富的苏氨酸，缬氨酸，异亮氨酸，亮氨酸和组氨酸。Lithovit^®- Amino25在生产平菇中具有很高的潜力。