Droplet microfluidics is a technology that enables the production and manipulation of small volumes. In biosciences, the most popular application of this technology is Droplet Digital™ PCR (ddPCR™), where parallel nanoliter-scale PCR assays are used to provide a high sensitivity and specificity for DNA detection. However, the recovery of PCR products for downstream applications such as sequencing can be challenging due to the droplets' stability. Here we compared five methods for disrupting the droplets to recover DNA. We found that rapid freezing in liquid nitrogen results in a clear phase separation and recovery of up to 70% of the DNA content. Liquid nitrogen freezing can thus offer a simple and environmentally friendly protocol for recovering DNA from ddPCR.

中文翻译：

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使用液氮从 Droplet Digital™ PCR 乳液中回收 DNA。

液滴微流体技术是一种能够生产和操作小体积的技术。在生物科学中，该技术最流行的应用是 Droplet Digital™ PCR (ddPCR™)，其中使用平行纳升级 PCR 检测为 DNA 检测提供高灵敏度和特异性。然而，由于液滴的稳定性，用于下游应用（如测序）的 PCR 产物的回收可能具有挑战性。在这里，我们比较了五种破坏液滴以恢复 DNA 的方法。我们发现在液氮中快速冷冻会导致清晰的相分离和高达 70% 的 DNA 含量的回收。因此，液氮冷冻可以为从 ddPCR 中回收 DNA 提供一种简单且环保的方案。