The aim of this study was to assess two protocols for their capacities to simultaneously isolate RNA, mtDNA and ncDNA from mammalian cells. We compared the Invitrogen TRIzol-based method and Qiagen DNeasy columns, using the HepG2 cell line and human primary glioblastoma stem cells. Both methods allowed the isolation of all three types of nucleic acids and provided similar yields in mtDNA. However, the yield in ncDNA was more than tenfold higher on columns, as observed for both cell types. Conversely, the TRIzol method proved more reproducible and was the method of choice for isolating RNA from glioblastoma cells, as demonstrated for the housekeeping genes RPLP0 and RPS9.

中文翻译：

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从哺乳动物细胞中同时定量分离线粒体 DNA、核 DNA 和 RNA 的方法。

本研究的目的是评估两种方案从哺乳动物细胞中同时分离 RNA、mtDNA 和 ncDNA 的能力。我们使用 HepG2 细胞系和人原代胶质母细胞瘤干细胞比较了基于 Invitrogen TRIzol 的方法和 Qiagen DNeasy 色谱柱。这两种方法都可以分离所有三种类型的核酸，并在 mtDNA 中提供相似的产量。然而，正如对两种细胞类型所观察到的那样，ncDNA 的产量在柱上高出十倍以上。相反，TRIzol 方法被证明具有更高的可重复性，并且是从胶质母细胞瘤细胞中分离 RNA 的首选方法，正如管家基因RPLP0和RPS9所证明的那样。