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Genomic characteristics and profile of microsatellite primers for Acanthogobius ommaturus by genome survey sequencing.
Bioscience Reports ( IF 4 ) Pub Date : 2020-10-28 , DOI: 10.1042/bsr20201295
Bingjie Chen 1 , Zhicheng Sun 1 , Fangrui Lou 1 , Tian-xiang Gao 2 , Na Song 1
Affiliation  

Acanthogobius ommaturus is one of the suitable species to study the genetic mechanism of adaptive evolution, but there are few reports on its genetics. In this study, the genomic survey sequencing method was used to analyze the genome characters of A. ommaturus. A total of 50.50 G high-quality sequence data was obtained in the present study. From the 19-mer distribution frequency, the estimated genome size was 928.01 Mb. The calculated sequence repeat rate was about 38.31%, the heterozygosity was about 0.17%, and the GC% content was about 40.88%. Moreover, 475,724 simple sequence repeats (SSRs) were identified. Among them, dinucleotide repeats were the most (53.70% of the total SSRs), followed by tri- (35.36%), hexa- (4.59%), tetra- (4.57%) and penta- (1.77%) nucleotide repeats type. This is the first genome-wide feature of this species to be reported.

中文翻译:

通过基因组调查测序确定Acanthogobius ommaturus微卫星引物的基因组特征和谱。

Acanthogobius ommaturus是研究适应性进化的遗传机制的合适物种之一,但有关其遗传学的报道很少。在这项研究中,基因组调查测序方法被用来分析A.omaturus的基因组特征。本研究共获得了50.50 G的高质量序列数据。从19-mer分布频率来看,估计的基因组大小为928.01 Mb。计算的序列重复率约为38.31%,杂合度约为0.17%,GC%含量约为40.88%。此外,鉴定了475,724个简单序列重复(SSR)。其中,二核苷酸重复序列最多(占总SSR的53.70%),其次是三核苷酸(35.36%),六核苷酸(4.59%),四核苷酸(4.57%)和五核苷酸(1.77%)。
更新日期:2020-10-31
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