We used unbiased next-generation sequencing (NGS) to detect unknown viruses in cats. Serum or plasma samples were obtained from clinically ill cats with suspected acute viral infections. Nucleic acid was extracted from serum or plasma samples to construct a complementary DNA library for NGS. Comprehensive nucleotide sequencing analyses enabled detection of the genomes of various viruses, including the severe fever with thrombocytopenia syndrome virus, feline immunodeficiency virus, feline morbillivirus, parvovirus, and Torque teno felis virus. Our findings indicate that comprehensive nucleotide analyses of serum or plasma samples can be used to detect infections with unknown viruses in cats.

我们使用无偏二代测序 (NGS) 来检测猫中的未知病毒。血清或血浆样本来自疑似急性病毒感染的临床病猫。从血清或血浆样品中提取核酸，构建 NGS 互补 DNA 文库。全面的核苷酸测序分析能够检测各种病毒的基因组，包括重症发热伴血小板减少综合征病毒、猫免疫缺陷病毒、猫麻疹病毒、细小病毒和 Torque teno 猫病毒。我们的研究结果表明，血清或血浆样本的综合核苷酸分析可用于检测猫的未知病毒感染。