Using microspherical scaffolds as building blocks to repair bone defects of specific size and shape has been proposed as a tissue engineering strategy. Here, phosphate glass (PG) microcarriers doped with 5 mol % TiO₂ and either 0 mol % CoO (CoO 0%) or 2 mol % CoO (CoO 2%) were investigated for their ability to support osteogenic and vascular responses of human mesenchymal stem cells (hMSCs). Together with standard culture techniques, cell-material interactions were studied using a novel perfusion microfluidic bioreactor that enabled cell culture on microspheres, along with automated processing and screening of culture variables. While titanium doping was found to support hMSCs expansion and differentiation, as well as endothelial cell-derived vessel formation, additional doping with cobalt did not improve the functionality of the microspheres. Furthermore, the microfluidic bioreactor enabled screening of culture parameters for cell culture on microspheres that could be potentially translated to a scaled-up system for tissue-engineered bone manufacturing.

已经提出使用微球形支架作为构建块来修复特定尺寸和形状的骨缺损作为组织工程策略。在此，掺杂有5 mol％TiO _2的磷酸盐玻璃（PG）微载体研究了0 mol％CoO（CoO 0％）或2 mol％CoO（CoO 2％）的能力，以支持人间充质干细胞（hMSCs）的成骨和血管反应。与标准的培养技术一起，使用新型的灌注微流体生物反应器研究了细胞与材料之间的相互作用，该反应器使细胞能够在微球上进行培养，并自动进行了培养变量的筛选和筛选。尽管发现钛掺杂可支持hMSC的扩增和分化，以及内皮细胞衍生的血管形成，但钴的其他掺杂并不能改善微球的功能。此外，微流生物反应器能够筛选用于在微球上进行细胞培养的培养参数，该参数可潜在地转化为用于组织工程化骨制造的放大系统。