Aim:

The present review aimed to consolidate and analyze the recent information about the use of zebrafish in studies concerning cisplatin-induced ototoxicity and otoprotection.

Material and methods:

The PubMed, Web of Science, and Scopus databanks were searched using the following MESH terms: zebrafish, cisplatin, ototoxicity. The identified publications were screened according to inclusion and exclusion criteria and the 26 qualifying manuscripts were included in the full-text analysis. The experimental protocols, including cisplatin concentrations, the exposure duration and the outcome measurements used in zebrafish larvae studies, were evaluated and the reported knowledge was summarized.

Results:

Twenty-six substances protecting from cisplatin-induced toxicity were identified with the use of zebrafish larvae. These substances include quinine, salvianolic acid B, berbamine 6, benzamil, quercetin, dexmedetomidine, dexamethsanone, quinoxaline, edaravone, apocynin, dimethyl sulfoxide, KR-22335, SRT1720, ORC-13661, 3-MA, D-methionine, mdivi-1, FUT-175, rapamycin, Z-LLF-CHO, ATX, NAC, CYM-5478, CHCP1, CHCP2 and leupeptin. The otoprotective effects of compounds were attributed to their anti-ROS, anti-apoptotic and cisplatin uptake-blocking properties. The broadest range of protection was achieved when the experimental flow used preconditioning with an otoprotective compound and later a co-incubation with cisplatin. Protection against a high concentration of cisplatin was observed only in protocols using short exposure times (4 and 6 h).

Conclusions:

The data extracted from the selected papers confirm that despite the differences between the human and the zebra fish hearing thresholds (as affected by cisplatin), the sensory cells of zebrafish and larval zebrafish are a valuable tool which could be used: (i) for the discovery of novel otoprotective substances and compounds; (ii) to screen their side effects and (iii) to extend the knowledge on the mechanisms of cisplatin-induced inner ear damage. For future studies, the development of a consensus experimental protocol is highly recommended.

中文翻译：

---

使用斑马鱼幼虫侧线研究对顺铂引起的耳毒性的保护作用：范围审查

目标：

本综述旨在巩固和分析有关在顺铂引起的耳毒性和耳保护研究中使用斑马鱼的最新信息。

材料与方法：

使用以下 MESH 术语搜索 PubMed、Web of Science 和 Scopus 数据库：斑马鱼、顺铂、耳毒性。根据纳入和排除标准筛选确定的出版物，并将 26 篇符合条件的手稿纳入全文分析。评估了实验方案，包括顺铂浓度、暴露持续时间和斑马鱼幼虫研究中使用的结果测量，并总结了报告的知识。

结果：

使用斑马鱼幼虫鉴定了 26 种防止顺铂引起的毒性的物质。这些物质包括奎宁、丹酚酸 B、小檗胺 6、苯扎米、槲皮素、右美托咪定、地塞米松、喹喔啉、依达拉奉、夹竹桃麻素、二甲亚砜、KR-22335、SRT1720、ORC-13661、3-MA、D-蛋氨酸、mdivi-1 、FUT-175、雷帕霉素、Z-LLF-CHO、ATX、NAC、CYM-5478、CHCP1、CHCP2 和亮肽素。化合物的耳保护作用归因于它们的抗活性氧、抗凋亡和顺铂摄取阻断特性。当实验流程使用耳保护化合物预处理，然后与顺铂共同孵育时，实现了最广泛的保护。仅在使用短暴露时间（4 和 6 小时）的方案中观察到对高浓度顺铂的保护作用。

结论：

从所选论文中提取的数据证实，尽管人类和斑马鱼的听力阈值存在差异（受顺铂的影响），但斑马鱼和幼虫斑马鱼的感觉细胞是一种有价值的工具，可用于：(i)发现新的耳保护物质和化合物；(ii) 筛选它们的副作用和 (iii) 扩展关于顺铂诱导的内耳损伤机制的知识。对于未来的研究，强烈建议制定共识实验方案。