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snRNA-seq reveals a subpopulation of adipocytes that regulates thermogenesis
Nature ( IF 64.8 ) Pub Date : 2020-10-28 , DOI: 10.1038/s41586-020-2856-x
Wenfei Sun 1 , Hua Dong 1 , Miroslav Balaz 1 , Michal Slyper 2 , Eugene Drokhlyansky 2 , Georgia Colleluori 3 , Antonio Giordano 3 , Zuzana Kovanicova 4 , Patrik Stefanicka 5 , Lucia Balazova 1 , Lianggong Ding 1 , Anna Sofie Husted 6 , Gottfried Rudofsky 7 , Jozef Ukropec 4 , Saverio Cinti 3 , Thue W Schwartz 6 , Aviv Regev 8, 9, 10 , Christian Wolfrum 1
Affiliation  

Adipose tissue is usually classified on the basis of its function as white, brown or beige (brite) 1 . It is an important regulator of systemic metabolism, as shown by the fact that dysfunctional adipose tissue in obesity leads to a variety of secondary metabolic complications 2 , 3 . In addition, adipose tissue functions as a signalling hub that regulates systemic metabolism through paracrine and endocrine signals 4 . Here we use single-nucleus RNA-sequencing (snRNA-seq) analysis in mice and humans to characterize adipocyte heterogeneity. We identify a rare subpopulation of adipocytes in mice that increases in abundance at higher temperatures, and we show that this subpopulation regulates the activity of neighbouring adipocytes through acetate-mediated modulation of their thermogenic capacity. Human adipose tissue contains higher numbers of cells of this subpopulation, which could explain the lower thermogenic activity of human compared to mouse adipose tissue and suggests that targeting this pathway could be used to restore thermogenic activity. Single-nucleus RNA sequencing in mouse and human adipose tissue identifies a subpopulation of adipocytes that regulates thermogenesis in neighbouring adipocytes in a paracrine manner by modulating acetate signalling.

中文翻译:

snRNA-seq揭示了调节产热的脂肪细胞亚群

脂肪组织通常根据其功能分为白色、棕色或米色 (brite) 1 。它是全身代谢的重要调节剂,如肥胖中功能失调的脂肪组织导致各种继发性代谢并发症 2 、 3 的事实所示。此外,脂肪组织作为信号枢纽,通过旁分泌和内分泌信号 4 调节全身代谢。在这里,我们在小鼠和人类中使用单核 RNA 测序 (snRNA-seq) 分析来表征脂肪细胞的异质性。我们在小鼠体内发现了一种罕见的脂肪细胞亚群,它们在较高温度下丰度增加,并且我们表明该亚群通过醋酸盐介导的产热能力调节来调节相邻脂肪细胞的活性。人类脂肪组织包含更多数量的该亚群细胞,这可以解释与小鼠脂肪组织相比人类的产热活性较低,并表明靶向该途径可用于恢复产热活性。小鼠和人类脂肪组织中的单核 RNA 测序鉴定了一个脂肪细胞亚群,该亚群通过调节醋酸信号以旁分泌方式调节相邻脂肪细胞的产热。
更新日期:2020-10-28
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