Dengue fever is endemic in more than 120 countries, which account for 3.9 billion people at risk of infection worldwide. The absence of a vaccine with effective protection against the four serotypes of this virus makes differential molecular diagnosis the key step for the correct treatment of the disease. Rapid and efficient diagnosis prevents progression to a more severe stage of this disease. Currently, the limiting factor in the manufacture of dengue (DENV) diagnostic kits is the lack of large-scale production of the non-structural 1 (NS1) protein (antigen) to be used in the capture of antibodies from the blood serum of infected patients. In this work, we use plant biotechnology and genetic engineering as tools for the study of protein production for research and commercial purposes. Gene transfer, integration and expression in plants is a valid strategy for obtaining large-scale and low-cost heterologous protein production. The authors produced NS1 protein of the dengue virus serotype 2 (NS1DENV2) in the Arabidopsis thaliana plant. Transgenic plants obtained by genetic transformation expressed the recombinant protein that was purified and characterized for diagnostic use. The yield was 203 μg of the recombinant protein per gram of fresh leaf. By in situ immunolocalization, transgenic protein was observed within the plant tissue, located in aggregates bodies. These antigens showed high sensitivity and specificity to both IgM (84.29% and 91.43%, respectively) and IgG (83.08% and 87.69%, respectively). The study goes a step further to validate the use of plants as a strategy for obtaining large-scale and efficient protein production to be used in dengue virus diagnostic tests.

登革热在120多个国家中是地方性流行，全世界有39亿人处于感染危险之中。缺乏能有效抵抗该病毒四种血清型的疫苗，使分子鉴别诊断成为正确治疗该疾病的关键步骤。快速有效的诊断可防止疾病进展至更严重的阶段。当前，制造登革热（DENV）诊断试剂盒的限制因素是缺乏大规模生产用于从感染者血清中捕获抗体的非结构性1（NS1）蛋白（抗原）的趋势。耐心。在这项工作中，我们使用植物生物技术和基因工程作为研究蛋白质生产的工具，以用于研究和商业目的。基因转移 植物中的整合和表达是获得大规模和低成本异源蛋白质生产的有效策略。作者在猪瘟中产生了登革病毒血清型2的NS1蛋白（NS1DENV2）。拟南芥厂。通过遗传转化获得的转基因植物表达了重组蛋白，该蛋白经过纯化和表征可用于诊断。每克新鲜叶的产量为203μg重组蛋白。通过原位免疫定位，在植物组织中观察到转基因蛋白，位于聚集体中。这些抗原对IgM（分别为84.29％和91.43％）和IgG（分别为83.08％和87.69％）都显示出高敏感性和特异性。这项研究进一步证明了使用植物作为获得大规模有效生产登革病毒诊断测试所需蛋白质的策略。