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Development and Characterization of EST-SSR Markers for Juniperus squamata (Cupressaceae), an ecologically important conifer in Asian mountains
Silvae Genetica ( IF 1 ) Pub Date : 2020-01-01 , DOI: 10.2478/sg-2020-0016
Tsam Ju 1 , Perla Farhat 1 , Wenjing Tao 1 , Jibin Miao 1 , Jialiang Li 1 , Xiaoyan Fan 1 , Sonam Tso 2 , Kangshan Mao 1
Affiliation  

Abstract Juniperus squamata, an endemic conifer of Asia, is an important shrub ecologically and economically. Yet little is known about its genetic diversity and population structure due to lacking of highly polymorphic molecular markers. In this study, expressed sequence tag microsatellite markers (EST-SSR) were developed for Juniperus squamata. Illumina HiSeq data were used to reconstruct the transcriptome of this species by de novo assembly. Based on this transcriptome, 18 SSR markers were designed and successfully amplified. Just one locus was eliminated due to its detection of null alleles and the remaining 17 loci were polymorphic, generating five to 14 alleles per locus in J. squamata. Markers cross-amplification tests were successful in two closely related species of J. squamata. These markers will serve as a basis for further studies to assess the genetic diversity and population structure of J. squamata. As well, they could be useful in promoting sustainable forest management strategies for this species in the face of global climate change.

中文翻译:

亚洲山区生态重要针叶树杜松(柏科)EST-SSR 标记的开发和表征

摘要 刺柏是亚洲特有的针叶树,在生态和经济上是重要的灌木。然而,由于缺乏高度多态性的分子标记,对其遗传多样性和种群结构知之甚少。在这项研究中,为杜松开发了表达序列标签微卫星标记(EST-SSR)。Illumina HiSeq 数据用于通过从头组装重建该物种的转录组。基于该转录组,设计并成功扩增了 18 个 SSR 标记。由于检测到无效等位基因,只有一个基因座被消除,其余 17 个基因座是多态的,在鳞状鳞癌中每个基因座产生 5 到 14 个等位基因。标记物交叉扩增试验在两个密切相关的鳞翅目物种中取得了成功。这些标记将作为进一步研究的基础,以评估鳞翅目的遗传多样性和种群结构。同样,面对全球气候变化,它们可能有助于促进该物种的可持续森林管理战略。
更新日期:2020-01-01
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