当前位置: X-MOL 学术Open Life Sci. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Therapeutic effects of coronary granulocyte colony-stimulating factor on rats with chronic ischemic heart disease
Open Life Sciences ( IF 2.2 ) Pub Date : 2020-10-20 , DOI: 10.1515/biol-2020-0078
Pengcheng Ren 1 , Ming Zhang 1 , Shuren Dai 1
Affiliation  

Abstract Background The aim of this study was to evaluate the therapeutic effects of coronary granulocyte colony-stimulating factor (G-CSF) on rats with chronic ischemic heart disease (CIHD). Methods Thirty healthy rats were randomly divided into control, subcutaneous and intracoronary G-CSF injection groups (n = 10) after the CIHD model was established. Left ventricular ejection fraction (LVEF), myocardial injury area, myocardial perfusion area and viable myocardium were observed by coronary angiography, dual-isotopic myocardial imaging and first-pass delayed myocardial perfusion magnetic resonance imaging (MRI) before modeling as well as 2 and 4 weeks after surgery. Results The peak times of peripheral blood and subcutaneous G-CSF levels were 3 and 5 days after mobilization, respectively. The peripheral blood CD34+/CD133+ cell ratio of subcutaneous or intracoronary G-CSF injection group significantly exceeded that of the control group (P < 0.05). The distal stenosis degrees of target lesions in subcutaneous and intracoronary G-CSF injection groups were significantly lower than that of the control group (P < 0.05). Compared with the situation before mobilization, LVEF was significantly improved after 2 weeks in intracoronary and subcutaneous G-CSF injection groups (P < 0.01). Their infarcted myocardial areas were reduced, the left ventricular remodeling was relieved, the percentage of viable myocardium was increased, angiogenesis was promoted and cardiomyocyte apoptosis was inhibited. Conclusion Intracoronary G-CSF injection is safe and effective as subcutaneous injection, improving the cardiac function of CIHD rats.

中文翻译:

冠脉粒细胞集落刺激因子对慢性缺血性心脏病大鼠的治疗作用

摘要 背景 本研究旨在评价冠状动脉粒细胞集落刺激因子(G-CSF)对慢性缺血性心脏病(CIHD)大鼠的治疗作用。方法 建立CIHD模型后,将30只健康大鼠随机分为对照组、皮下注射组和冠状动脉内注射G-CSF组(n=10)。造模前及2、4用冠状动脉造影、双同位素心肌显像、首过延迟心肌灌注磁共振成像(MRI)观察左心室射血分数(LVEF)、心肌损伤面积、心肌灌注面积和存活心肌手术后几周。结果外周血和皮下G-CSF水平的高峰时间分别为动员后3天和5天。皮下或冠脉G-CSF注射组外周血CD34+/CD133+细胞比值明显高于对照组(P < 0.05)。皮下及冠脉G-CSF注射组靶病灶远端狭窄程度明显低于对照组(P < 0.05)。与动员前相比,冠脉内和皮下注射G-CSF组2周后LVEF明显改善(P < 0.01)。其梗死心肌面积减少,左心室重构减轻,存活心肌百分比增加,促进血管生成,抑制心肌细胞凋亡。结论冠状动脉内注射G-CSF与皮下注射一样安全有效,可改善CIHD大鼠的心功能。
更新日期:2020-10-20
down
wechat
bug