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Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species
Journal of Reproduction and Development ( IF 1.8 ) Pub Date : 2020-01-01 , DOI: 10.1262/jrd.2020-086
Yan-Xia Peng 1 , Cheng-Zhen Chen 1 , Dan Luo 1 , Wen-Jie Yu 1 , Sheng-Peng Li 1 , Yue Xiao 1 , Bao Yuan 1 , Shuang Liang 1 , Xue-Rui Yao 2 , Nam-Hyung Kim 1, 2 , Hao Jiang 1, 2 , Jia-Bao Zhang 1
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Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added CA to parthenogenetically activated porcine embryos in an in vitro culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial membrane potential, and embryonic development-related gene expression. The results showed that supplementation with 10 μM CA during in vitro culture significantly improved the cleavage rates, blastocyst formation rates, hatching rates, and total numbers of cells of parthenogenetically activated porcine embryos compared with no supplementation. More importantly, supplementation with CA also improved GSH levels and mitochondrial membrane potential, reduced natural ROS levels in blastomeres, upregulated Nanog, Sox2, Gata4, Cox2, Itga5, and Rictor expression, and downregulated Birc5 and Caspase3 expression. These results suggest that CA can improve early porcine embryonic development by regulating oxidative stress. This study elucidates the effects of CA on early embryonic development and their potential mechanisms, and provides new applications for improving the quality of in vitro-developed embryos.

中文翻译:

鼠尾草酸通过抑制活性氧的积累来改善猪的早期胚胎发育

鼠尾草酸 (CA) 是一种天然儿茶酚松香二萜,用作动物饲料和人类食品中的添加剂。然而,CA 对哺乳动物生殖过程,尤其是早期胚胎发育的影响尚不清楚。在这项研究中,我们将 CA 添加到体外培养基中孤雌生殖激活的猪胚胎中,以探讨 CA​​ 对细胞凋亡、增殖、囊胚形成、活性氧 (ROS) 水平、谷胱甘肽 (GSH) 水平、线粒体膜电位、和胚胎发育相关的基因表达。结果表明,与未添加相比,在体外培养过程中添加 10 μM C​​A 显着提高了孤雌生殖活化猪胚胎的卵裂率、胚泡形成率、孵化率和细胞总数。更重要的是,补充 CA 还提高了 GSH 水平和线粒体膜电位,降低了卵裂球中的天然 ROS 水平,上调了 Nanog、Sox2、Gata4、Cox2、Itga5 和 Rictor 的表达,并下调了 Birc5 和 Caspase3 的表达。这些结果表明CA可以通过调节氧化应激来改善早期猪胚胎发育。该研究阐明了CA对早期胚胎发育的影响及其潜在机制,为提高体外发育胚胎的质量提供了新的应用。这些结果表明CA可以通过调节氧化应激来改善早期猪胚胎发育。该研究阐明了CA对早期胚胎发育的影响及其潜在机制,为提高体外发育胚胎的质量提供了新的应用。这些结果表明CA可以通过调节氧化应激来改善早期猪胚胎发育。该研究阐明了CA对早期胚胎发育的影响及其潜在机制,为提高体外发育胚胎的质量提供了新的应用。
更新日期:2020-01-01
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