当前位置: X-MOL 学术Cell Death Differ. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Maternally inherited intron coordinates primordial germ cell homeostasis during Drosophila embryogenesis
Cell Death and Differentiation ( IF 12.4 ) Pub Date : 2020-10-22 , DOI: 10.1038/s41418-020-00642-6
Ismail Osman 1, 2 , Jun Wei Pek 1, 2
Affiliation  

Primordial germ cells (PGCs) give rise to the germline stem cells (GSCs) in the adult Drosophila gonads. Both PGCs and GSCs need to be tightly regulated to safeguard the survival of the entire species. During larval development, a non-cell autonomous homeostatic mechanism is in place to maintain PGC number in the gonads. Whether such germline homeostasis occurs during early embryogenesis before PGCs reach the gonads remains unclear. We have previously shown that the maternally deposited sisRNA sisR-2 can influence GSC number in the female progeny. Here we uncover the presence of a homeostatic mechanism regulating PGCs during embryogenesis. sisR-2 represses PGC number by promoting PGC death. Surprisingly, increasing maternal sisR-2 leads to an increase in PGC death, but no drop in PGC number was observed. This is due to ectopic division of PGCs via the de-repression of Cyclin B, which is governed by a genetic pathway involving sisR-2, bantam and brat. We propose a cell autonomous model whereby germline homeostasis is achieved by preserving PGC number during embryogenesis.



中文翻译:

母系遗传的内含子在果蝇胚胎发生过程中协调原始生殖细胞稳态

原始生殖细胞 (PGC) 在成年果蝇性腺中产生生殖干细胞 (GSC)。PGCs 和 GSCs 都需要受到严格监管,以保障整个物种的生存。在幼虫发育过程中,有一种非细胞自主稳态机制来维持性腺中的 PGC 数量。在 PGC 到达性腺之前的早期胚胎发育过程中是否存在这种种系稳态仍不清楚。我们之前已经表明,母体沉积的 sisRNA sisR-2可以影响雌性后代的 GSC 数量。在这里,我们揭示了在胚胎发生过程中调节 PGCs 的稳态机制的存在。sisR-2通过促进 PGC 死亡来抑制 PGC 数量。令人惊讶的是,增加母体sisR-2导致PGC死亡增加,但未观察到PGC数量下降。这是由于 PGC 通过 Cyclin B 的去抑制而异位分裂所致,Cyclin B 由涉及sisR-2、bantambrat的遗传途径控制。我们提出了一种细胞自主模型,通过在胚胎发生过程中保留 PGC 数来实现种系稳态。

更新日期:2020-10-28
down
wechat
bug