The genomes of DNA tumor viruses regain nuclear localization after nuclear envelope breakdown during mitosis through the action of a viral protein with a chromatin-tethering domain (CTD). Here, we report that the human cytomegalovirus (HCMV) genome is maintained during mitosis by the CTD of the viral IE19 protein. Deletion of the IE19 CTD or disruption of the IE19 splice acceptor site reduced viral genome maintenance and progeny virion formation during infection of dividing fibroblasts, both of which were rescued by IE19 ectopic expression. The discovery of a viral genome maintenance factor during productive infection provides new insight into the mode of HCMV infection implicated in birth defects, organ transplant failure, and cancer.

中文翻译：

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人类巨细胞病毒基因组通过IE19染色质束缚域生存有丝分裂

DNA肿瘤病毒的基因组在有丝分裂期间通过包被染色质束缚域（CTD）的病毒蛋白的作用而在核被膜破裂后重新获得核定位。在这里，我们报告人类巨细胞病毒（HCMV）基因组在有丝分裂期间通过病毒IE19蛋白的CTD得以维持。IE19 CTD的删除或IE19剪接受体位点的破坏减少了分裂成纤维细胞感染期间的病毒基因组维持和子代病毒体的形成，两者均通过IE19异位表达得以挽救。生产性感染期间病毒基因组维持因子的发现为与出生缺陷，器官移植失败和癌症有关的HCMV感染模式提供了新见解。