Endophyte bestows beneficial aspects to its inhabiting host, along with a contribution to diverse structural attributes with biological potential. In this regard, antimicrobial profiling of fungal endophytes from medicinal plant Adiantum philippense revealed bioactive Nigrospora sphaerica from the leaf segment. Chemical and biological profiling through TLC–bioautography and hyphenated spectroscopic techniques confirmed the presence of phomalactone as an antimicrobial metabolite. The chemical investigation of the broth extract by bioassay-guided fractionation confirmed phomalactone as a bioactive antimicrobial secondary metabolite. The antimicrobial activity of phomalactone was found to be highest against Escherichia coli by disc diffusion assay. The MIC was found to be significant against both Escherichia coli and Xanthomonas campestris in the case of bacteria and dermatophyte Candida albicans at 150 μg/ml, respectively. Overall, the results highlighted the antimicrobial potential of phomalactone from the endophyte Nigrospora sphaerica exhibiting a broad spectrum of antimicrobial activity against human and phytopathogenic bacteria and fungi. This work is the first report regarding the antibacterial activity of phomalactone.

中文翻译：

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抗菌代谢谱球形黑孢从半月形铁线蕨L.

内生菌赋予其寄主宿主有益的方面，以及对具有生物学潜力的各种结构特征的贡献。在这方面，来自药用植物铁线蕨的真菌内生菌的抗菌谱分析显示了叶节段具有生物活性的黑夜孢子（Nigrospora sphaerica）。通过TLC-生物医学和联用光谱技术进行的化学和生物学分析证实了磷内酯是抗微生物代谢产物。通过生物测定指导的分级分离法对肉汤提取物进行化学研究，确认了膦内酯是一种具有生物活性的抗微生物次生代谢产物。通过盘片扩散测定法发现，膦内酯对大肠杆菌的抗菌活性最高。发现对于细菌和皮肤癣菌念珠菌念珠菌（Candida albicans）分别为150μg/ ml而言，MIC对大肠杆菌和野油菜黄单胞菌均具有重要意义。总体而言，该结果突出了内生菌Nigrospora sphaerica的磷内酯的抗菌潜力，对人类和植物致病性细菌和真菌具有广泛的抗菌活性。这项工作是有关phomalactone的抗菌活性的第一个报告。