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Exploration of in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic potentials with phytochemical screening of flowers of Brassica nigra
Future Journal of Pharmaceutical Sciences Pub Date : 2020-10-22 , DOI: 10.1186/s43094-020-00099-x
Mohammad Sarowar Uddin , Md. Shalahuddin Millat , Mohammad Safiqul Islam , Md. Saddam Hussain , Md. Giash Uddin , Shafayet Ahmed Siddiqui , Mahmuda Ferdous

Brassica nigra is a plant of Brassicaceae family, which possesses numerous medicinal values. Our present study is intended to assess the potential in vitro thrombolytic, anthelminthic, cytotoxic and in vivo anxiolytic properties of MCE of B. nigra flowers. MCE was fractioned for separating the compound on the basis of polarity by using chloroform, n-hexane and ethyl acetate solvent. Thrombolytic and anthelminthic activities were explained by collecting human erythrocytes and earthworms as test models, respectively. Anxiolytic activity was evaluated by elevated plus maze and hole board models while cytotoxic test was conducted through brine shrimp lethality bioassay. MCE revealed the presence of alkaloids, flavonoids, tannin, diterpenes, glycosides, carbohydrates, phenols, fixed oils and fat. In case of thrombolytic test, the MCE, CSF, ASF and n-HSF had produced maximum clot lysis activity at 5 and 10 mg/ml dose conditions. Two different concentrations (10 and 20 mg/ml) of MCE and its fractions showed significant (p < 0.05) anthelminthic activities in a dose-dependent manner. Significant anxiolytic activity was observed for all fractions which was comparable to the standard drug diazepam (p < 0.05). Again, the cytotoxic screening also presented good potentials for all fractions. From the findings of present study, we can conclude that MCE of B. nigra flowers and its fraction possess significant anxiolytic, anthelmintic, anticancer and thrombolytic properties which may be a good candidate for treating these diseases through the determination of bio-active lead compounds.

中文翻译:

芸chemical属植物花的植物化学筛选探索体外溶栓,驱虫,驱虫,细胞毒性和体内抗焦虑的潜力

黑芸甘蓝是芸苔科的一种植物,具有许多药用价值。我们目前的研究旨在评估黑芥子花MCE的体外溶栓,驱虫,驱虫,细胞毒性和体内抗焦虑特性。通过使用氯仿,正己烷和乙酸乙酯溶剂,分馏MCE以基于极性分离化合物。通过分别收集人类红细胞和earth作为测试模型来解释溶栓和驱虫活性。通过高架迷宫和孔板模型评估抗焦虑活性,同时通过盐水虾致死率生物测定法进行细胞毒性测试。MCE显示存在生物碱,类黄酮,单宁,二萜,糖苷,碳水化合物,酚,不挥发性油和脂肪。如果是溶栓试验,则MCE,CSF,ASF和n-HSF在5和10 mg / ml剂量条件下产生了最大的凝块溶解活性。两种不同浓度(10和20 mg / ml)的MCE及其馏分以剂量依赖性方式显示出显着的(p <0.05)驱虫活性。在所有馏分中均观察到明显的抗焦虑活性,与标准药物地西epa相当(p <0.05)。再次,细胞毒性筛选对于所有部分也显示出良好的潜力。从本研究的发现,我们可以得出结论,黑芥菜花及其部分的MCE具有显着的抗焦虑,驱虫,抗癌和血栓溶解特性,通过测定生物活性先导化合物可能是治疗这些疾病的良好候选者。两种不同浓度(10和20 mg / ml)的MCE及其馏分以剂量依赖性方式显示出显着的(p <0.05)驱虫活性。在所有馏分中均观察到明显的抗焦虑活性,与标准药物地西epa相当(p <0.05)。再次,细胞毒性筛选对于所有部分也显示出良好的潜力。从本研究的发现,我们可以得出结论,黑芥菜花及其部分的MCE具有显着的抗焦虑,驱虫,抗癌和血栓溶解特性,通过测定生物活性先导化合物可能是治疗这些疾病的良好候选者。两种不同浓度(10和20 mg / ml)的MCE及其馏分以剂量依赖性方式显示出显着的(p <0.05)驱虫活性。在所有馏分中均观察到明显的抗焦虑活性,与标准药物地西epa相当(p <0.05)。再次,细胞毒性筛选对于所有部分也显示出良好的潜力。从本研究的发现,我们可以得出结论,黑芥菜花及其部分的MCE具有显着的抗焦虑,驱虫,抗癌和血栓溶解特性,通过测定生物活性先导化合物可能是治疗这些疾病的良好候选者。在所有馏分中均观察到明显的抗焦虑活性,与标准药物地西epa相当(p <0.05)。再次,细胞毒性筛选对于所有部分也显示出良好的潜力。从本研究的发现,我们可以得出结论,黑芥菜花及其部分的MCE具有显着的抗焦虑,驱虫,抗癌和血栓溶解特性,通过测定生物活性先导化合物可能是治疗这些疾病的良好候选者。在所有馏分中均观察到明显的抗焦虑活性,与标准药物地西epa相当(p <0.05)。再次,细胞毒性筛选对于所有部分也显示出良好的潜力。从本研究的发现,我们可以得出结论,黑芥菜花及其部分的MCE具有显着的抗焦虑,驱虫,抗癌和血栓溶解特性,通过测定生物活性先导化合物可能是治疗这些疾病的良好候选者。
更新日期:2020-10-27
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