Deep vein thrombosis (DVT) is associated with stroke. Here, we hypothesize that genes associated with DVT may also play roles in the development of stroke. we firstly conducted large-scale literature based disease-gene relationship data analysis to explore the genes implicated with DVT and stroke. Further, a mega-analysis was conducted for each of these genes that were linked to DVT but not stroke, using 11 independent stroke RNA expression datasets (176 stroke cases and 102 healthy controls). Then, a multiple linear regression (MLR) model was employed to study possible influential factors on the gene expression levels in stroke. After that, a functional pathway analysis was performed to identify the potential biological linkage between stroke and the target genes suggested by mega-analysis. Over 81.10% genes implicated with DVT also suggested an association with stroke. Among the 24 DVT-specific genes, one DVT-inhibiting gene, SP1, presented significantly increased expression in stroke (LFC = 1.34, p-value = 0.0045). Pathway analysis showed that SP1 may play a therapeutic role in post-stroke patients by promoting multiple of stroke-inhibitors. Moreover, geographical region was indicated as an influential factor on the expression levels of SP1 in stroke samples (p-value = 0.037). Our results suggested that DVT inhibitor SP1 could be a novel therapeutic target gene for post-stroke treatment. Further study of the potential relations between SP1 and stroke was guaranteed.

中文翻译：

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深静脉血栓形成抑制剂可能在中风后患者中发挥治疗作用

深静脉血栓形成（DVT）与中风有关。在这里，我们假设与DVT相关的基因也可能在中风的发展中发挥作用。我们首先进行了基于文献的大规模疾病-基因关系数据分析，以探讨与DVT和中风有关的基因。此外，使用11个独立的中风RNA表达数据集（176个中风病例和102个健康对照），对与DVT关联但与中风无关的每个基因进行了大型分析。然后，采用多元线性回归（MLR）模型研究脑卒中基因表达水平的可能影响因素。之后，进行功能通路分析，以鉴定中风与大型分析提示的靶基因之间的潜在生物学联系。超过81。与DVT有关的基因中有10％也暗示与中风有关。在24种DVT特异性基因中，一种DVT抑制基因SP1表示中风的表达显着增加（LFC = 1.34，p值= 0.0045）。路径分析表明，SP1可能通过促进多种中风抑制剂而在中风后患者中发挥治疗作用。而且，地理区域被指示为中风样品中SP1表达水平的影响因素（p值＝ 0.037）。我们的结果表明，DVT抑制剂SP1可能是中风后治疗的新型治疗靶基因。保证了对SP1和中风之间潜在关系的进一步研究。路径分析表明，SP1可能通过促进多种中风抑制剂而在中风后患者中发挥治疗作用。而且，地理区域被指示为中风样品中SP1表达水平的影响因素（p值＝ 0.037）。我们的结果表明，DVT抑制剂SP1可能是中风后治疗的新型治疗靶基因。保证了对SP1和中风之间潜在关系的进一步研究。路径分析表明，SP1可能通过促进多种中风抑制剂而在中风后患者中发挥治疗作用。而且，地理区域被指示为中风样品中SP1表达水平的影响因素（p值＝ 0.037）。我们的结果表明，DVT抑制剂SP1可能是中风后治疗的新型治疗靶基因。保证了对SP1和中风之间潜在关系的进一步研究。