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Synthetic model ecosystem of 12 cryopreservable microbial species allowing for a noninvasive approach
bioRxiv - Microbiology Pub Date : 2023-10-31 , DOI: 10.1101/2020.10.23.351742
Kazufumi Hosoda , Shigeto Seno , Naomi Murakami , Hideo Matsuda , Yutaka Osada , Michio Kondoh

Simultaneous understanding of both individual and ecosystem dynamics is crucial in an era marked by the degradation of ecosystem services. Herein, we present a high-throughput synthetic microcosm system comprising 12 functionally and phylogenetically diverse microbial species. These species are axenically culturable, cryopreservable, and can be measured noninvasively via microscopy, aided by machine learning. This system includes prokaryotic and eukaryotic producers and decomposers, and eukaryotic consumers to ensure functional redundancy. Our model system displayed both positive and negative interspecific interactions and higher-order interactions that surpassed the scope of any two-species interaction. Although complete species coexistence was not our primary objective, we identified several conditions under which at least one species from the producers, consumers, and decomposers groups, and one functionally redundant species, persisted for over six months. These conditions set the stage for detailed investigations in the future. Given its designability and experimental replicability, our model ecosystem offers a promising platform for deeper insights into both individual and ecosystem dynamics, including evolution and species interactions.

中文翻译:

12 种可低温保存微生物物种的综合模型生态系统,可实现非侵入性方法

在生态系统服务退化的时代,同时了解个体和生态系统的动态至关重要。在此,我们提出了一个高通量合成微观世界系统,包含 12 种功能和系统发育多样化的微生物物种。这些物种可以进行无菌培养、冷冻保存,并且可以在机器学习的辅助下通过显微镜进行非侵入性测量。该系统包括原核和真核生产者和分解者以及真核消费者以确保功能冗余。我们的模型系统显示了积极和消极的种间相互作用以及超出任何两个物种相互作用范围的高阶相互作用。尽管完整的物种共存不是我们的主要目标,但我们确定了几种条件,在这些条件下,至少一种来自生产者、消费者和分解者群体的物种以及一种功能冗余的物种持续存在超过六个月。这些条件为将来的详细调查奠定了基础。鉴于其可设计性和实验可复制性,我们的模型生态系统提供了一个有前途的平台,可以更深入地了解个体和生态系统的动态,包括进化和物种相互作用。
更新日期:2023-11-01
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