BACKGROUND Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was confirmed in Brazil in February 2020, the first cases were followed by an increase in the number of cases throughout the country, resulting in an important public health crisis that requires fast and coordinated responses. OBJECTIVES The objective of this work is to describe the isolation and propagation properties of SARS-CoV-2 isolates from the first confirmed cases of coronavirus disease 2019 (COVID-19) in Brazil. METHODS After diagnosis in patients that returned from Italy to the São Paulo city in late February by RT-PCR, SARS-CoV-2 isolates were obtained in cell cultures and characterised by full genome sequencing, electron microscopy and in vitro replication properties. FINDINGS The virus isolate was recovered from nasopharyngeal specimen, propagated in Vero cells (E6, CCL-81 and hSLAM), with clear cytopathic effects, and characterised by full genome sequencing, electron microscopy and in vitro replication properties. Virus stocks - viable (titre 2.11 × 106 TCID50/mL, titre 1.5 × 106 PFUs/mL) and inactivated from isolate SARS.CoV2/SP02.2020.HIAE.Br were prepared and set available to the public health authorities and the scientific community in Brazil and abroad. MAIN CONCLUSION We believe that the protocols for virus growth and studies here described and the distribution initiative may constitute a viable model for other developing countries, not only to help a rapid effective pandemic response, but also to facilitate and support basic scientific research.

中文翻译：

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从巴西首批报告的患者中分离出 SARS-CoV-2 并建立协调的任务网络

背景 2020 年 2 月，巴西确认了严重急性呼吸系统综合症冠状病毒 2（SARS-CoV-2），首例病例随后在全国范围内增加，导致严重的公共卫生危机，需要迅速和协调响应。目的 这项工作的目的是描述从巴西首例确诊的 2019 年冠状病毒病 (COVID-19) 病例中分离出的 SARS-CoV-2 分离株和传播特性。方法 2 月下旬从意大利返回圣保罗市的患者通过 RT-PCR 诊断后，在细胞培养物中获得 SARS-CoV-2 分离株，并通过全基因组测序、电子显微镜和体外复制特性进行表征。结果 从鼻咽标本中分离出病毒分离物，在 Vero 细胞（E6、CCL-81 和 hSLAM）中繁殖，具有明显的细胞病变效应，并具有全基因组测序、电子显微镜和体外复制特性。病毒储备 - 可行（滴度 2.11 × 106 TCID50/mL，滴度 1.5 × 106 PFUs/mL）和从分离株 SARS.CoV2/SP02.2020.HIAE.Br 中灭活的病毒已制备并可供公共卫生当局和科学界使用在巴西和国外。主要结 电子显微镜和体外复制特性。病毒储备 - 可行（滴度 2.11 × 106 TCID50/mL，滴度 1.5 × 106 PFUs/mL）和从分离株 SARS.CoV2/SP02.2020.HIAE.Br 中灭活的病毒已制备并可供公共卫生当局和科学界使用在巴西和国外。主要结 电子显微镜和体外复制特性。病毒储备 - 可行（滴度 2.11 × 106 TCID50/mL，滴度 1.5 × 106 PFUs/mL）和从分离株 SARS.CoV2/SP02.2020.HIAE.Br 中灭活的病毒已制备并可供公共卫生当局和科学界使用在巴西和国外。主要结 Br 已准备好并可供巴西和国外的公共卫生当局和科学界使用。主要结 Br 已准备好并可供巴西和国外的公共卫生当局和科学界使用。主要结