In this work, the peroxidase-like properties of dendritic Au-Pt nanoparticles (30~60 nm) were investigated and these nanoparticles were applied in the immunoassay as signal labels for the detection of capsular polysaccharides (CPS, ~ 300 kDa, expressed on the cell envelopes of Burkholderia pseudomallei causing melioidosis). The immunoassay adopts monoclonal anti-CPS antibodies. The assay limit of detection (LOD), which is calculated through the 3σ method, is at 0.36 ng/mL for CPS spiked in PBS with 5% milk, and at 0.88 ng/mL for CPS spiked in human serum. The results from this work show that the dendritic Au-Pt nanoparticles can produce similar assay performance in detecting CPS as HRP did. Considering these particles can be easily prepared and stable in environments (thus avoiding cold-chain based storage/transportation), they have potential to be applicable for both in-house and in-field immunoassay development for the pathogen B. pseudomallei detection.

在这项工作中，研究了树突状Au-Pt纳米粒子（30〜60 nm）的过氧化物酶样性质，并将这些纳米粒子用于免疫测定，作为检测荚膜多糖（CPS，〜300 kDa，表达在胶囊上）的信号标记。假伯克霍尔德氏菌细胞包膜造成类li虫病）。免疫测定采用单克隆抗CPS抗体。通过3σ方法计算的检测限（LOD），对于加标有5％牛奶的PBS中的CPS为0.36 ng / mL，对人血清中加标的CPS为0.88 ng / mL。这项工作的结果表明，树突状的Au-Pt纳米颗粒可以在检测CPS方面产生与HRP相似的测定性能。考虑到这些颗粒易于制备且在环境中稳定（因此避免了基于冷链的存储/运输），因此它们有潜力可用于病原体假性疟原虫检测的内部和现场免疫分析开发。