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Proteomics Study of DNA–Protein Crosslinks in Methylmethanesulfonate and Fe2+-EDTA-Exposed Human Cells
Chemical Research in Toxicology ( IF 4.1 ) Pub Date : 2020-10-26 , DOI: 10.1021/acs.chemrestox.0c00289
Yat-Hing Ham 1 , K K Jason Chan 1 , Dominik Madej 2 , Henry Lam 2 , Wan Chan 1
Affiliation  

The formation of covalently bound DNA–protein crosslinks (DPCs) is linked to the pathophysiology of cancers and many other degenerative diseases. Knowledge of the proteins that were frequently involved in forming DPCs will improve our understanding of the etiological mechanism of diseases and facilitate the establishment of preventive measures and treatment methods. By using SDS-PAGE and nano-LC coupled Orbitrap LC-MS/MS analyses, we identified, for the first time, that the major DNA-cross-linked proteins in HeLa cells exposed to a methylating agent (methylmethanesulfonate) or hydroxyl free radicals are transcription-associated proteins. In particular, histone H2B3B and poly(rC) binding protein 2 were identified as the most frequent DPC-forming proteins.

中文翻译:

甲基磺酸盐和 Fe2+-EDTA 暴露的人类细胞中 DNA-蛋白质交联的蛋白质组学研究

共价结合的 DNA-蛋白质交联 (DPC) 的形成与癌症和许多其他退行性疾病的病理生理学有关。了解经常参与形成 DPC 的蛋白质将提高我们对疾病病因机制的理解,并有助于制定预防措施和治疗方法。通过使用 SDS-PAGE 和纳米 LC 耦合 Orbitrap LC-MS/MS 分析,我们首次确定了 HeLa 细胞中暴露于甲基化剂(甲基甲磺酸盐)或羟基自由基的主要 DNA 交联蛋白是转录相关蛋白。特别是,组蛋白 H2B3B 和 poly(rC) 结合蛋白 2 被确定为最常见的 DPC 形成蛋白。
更新日期:2020-11-16
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