Long non-coding RNA Plasmacytoma Variant Translocation 1 (LncRNA PVT1) was involved in various human diseases, but its role in aortic dissection (AD) remained to be fully examined. In this study, the viability and migration of human aortic smooth muscle cells (HASMCs) were respectively measured by MTT assay and wound-healing assay. Relative phenotypic switch-related protein expressions were measured with quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as needed. An AD model was established in animals and hematoxylin–eosin (H&E) staining was used for pathological examination. We found that, in HASMCs, microRNA (miR)-27b-3p could competitively bind with PVT1. In AD, PVT1 expression was upregulated, yet that of miR-27b-3p was downregulated. Downregulating PVT1 reversed the effects of growth factor-BB (PDGF-BB) treatment on PVT1, miR-27b-3p and expressions of phenotypic switch-related markers, and cell viability and migration, while downregulating miR-27b-3p reversed the effects of downregulating PVT1. Moreover, downregulating PVT1 suppressed the effects of upregulated PVT1 and downregulated miR-27b-3p induced by AD as well as media degeneration in vivo. In conclusion, downregulating PVT1 expression suppressed the proliferation, migration and phenotypic switch of HASMCs treated by PDGF-BB via targeting miR-27b-3p.

长链非编码 RNA 浆细胞瘤变体易位 1 (LncRNA PVT1) 与多种人类疾病有关，但其在主动脉夹层 (AD) 中的作用仍有待充分研究。在这项研究中，人主动脉平滑肌细胞 (HASMCs) 的活力和迁移分别通过 MTT 法和伤口愈合法测量。根据需要使用定量实时聚合酶链反应 (qRT-PCR) 和蛋白质印迹测量相对表型开关相关蛋白表达。在动物中建立AD模型并使用苏木精-伊红（H＆E）染色进行病理检查。我们发现，在 HASMC 中，microRNA (miR)-27b-3p 可以与 PVT1 竞争性结合。在 AD 中，PVT1 表达上调，而 miR-27b-3p 表达下调。下调 PVT1 可逆转生长因子-BB (PDGF-BB) 处理对 PVT1、miR-27b-3p 和表型转换相关标志物的表达以及细胞活力和迁移的影响，而下调 miR-27b-3p 可逆转下调 PVT1。此外，下调 PVT1 抑制了由 AD 诱导的上调 PVT1 和下调 miR-27b-3p 以及体内培养基变性的影响。总之，下调 PVT1 表达可通过靶向 miR-27b-3p 抑制经 PDGF-BB 处理的 HASMC 的增殖、迁移和表型转换。下调 PVT1 抑制了由 AD 诱导的上调 PVT1 和下调 miR-27b-3p 以及体内培养基变性的影响。总之，下调 PVT1 表达可通过靶向 miR-27b-3p 抑制经 PDGF-BB 处理的 HASMC 的增殖、迁移和表型转换。下调 PVT1 抑制了由 AD 诱导的上调 PVT1 和下调 miR-27b-3p 以及体内培养基变性的影响。总之，下调 PVT1 表达可通过靶向 miR-27b-3p 抑制经 PDGF-BB 处理的 HASMC 的增殖、迁移和表型转换。