Abstract

Polycaprolactone diol is the cornerstone, equipped with polyacrylonitrile and cellulose nanowhiskers (CNWs), of biocompatible and biodegradable polyurethanes (PUs). The solvent casting/particulate leaching technique was employed to contracting foam scaffolds with bimodal sizes from the combination of polyurethane/polyacrylonitrile/cellulose nanowhisker nanocomposites. Sugar and sodium chloride are components used as porogens to develop the leaching method and fabricate the 3D scaffolds. Incorporation of different percentages of cellulose nanowhisker leads to the various efficient structures with biodegradability and biocompatibility properties. All nanocomposites scaffolds, as revealed by MTT assay using mesenchymal stem cell (MSC) lines, were non-cytotoxic. PU/PAN/CNW foam scaffolds were used for osteogenic differentiation of human mesenchymal stem cells (hMSCs). Based on the results, PU/PAN/CNW nanocomposites could not only support osteogenic differentiation but can also enhance the proliferation of hMSCs in three-dimensional synthetic extracellular matrix.

Graphic abstract

中文翻译：

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间充质干细胞在含有磷酸纤维素纳米晶须的双峰聚合物聚氨酯/聚丙烯腈上的成骨分化

摘要

聚己内酯二醇是生物相容性和可生物降解聚氨酯 (PU) 的基石，配备聚丙烯腈和纤维素纳米晶须 (CNW)。采用溶剂浇铸/颗粒浸出技术，从聚氨酯/聚丙烯腈/纤维素纳米晶须纳米复合材料的组合中收缩具有双峰尺寸的泡沫支架。糖和氯化钠是用作致孔剂以开发浸出方法和制造 3D 支架的成分。掺入不同百分比的纤维素纳米晶须导致具有生物降解性和生物相容性的各种有效结构。正如使用间充质干细胞 (MSC) 系的 MTT 测定所揭示的，所有纳米复合材料支架都是无细胞毒性的。PU/PAN/CNW 泡沫支架用于人类间充质干细胞 (hMSCs) 的成骨分化。结果表明，PU/PAN/CNW纳米复合材料不仅可以支持成骨分化，还可以增强hMSCs在三维合成细胞外基质中的增殖。

图形摘要