Cationic amino acid transporter 1 (Cat-1 alias Slc7a1) is a Na⁺-independent carrier system involved in transport and absorption of the cationic amino acids lysine, arginine, histidine, and ornithine and has also been shown to be indispensable in a large variety of biological processes. Starting from isolated full-length zebrafish (Danio rerio) cDNA for slc7a1a, we performed comparative and phylogenetic sequence analysis, investigated the conservation of the gene during vertebrate evolution, and defined tissue expression during zebrafish development. Whole mount in situ hybridization first detected slc7a1a transcripts in somites, eyes, and brain at 14 h post-fertilization (hpf) with additional expression in the distal nephron at 24 hpf and in branchial arches at 3 days post-fertilization (dpf), with significant increase by 5 dpf. Taken together, the expression analysis of the zebrafish Cat-1 system gene slc7a1a suggests a functional role(s) during the early development of the central nervous system, muscle, gills, and kidney.

阳离子氨基酸转运蛋白1（Cat-1别名Slc7a1）是不依赖Na ^+的载体系统，参与阳离子氨基酸赖氨酸，精氨酸，组氨酸和鸟氨酸的转运和吸收，并且已被证明在许多品种中都是必不可少的生物过程。从slc7a1a的分离的全长斑马鱼（Danio rerio）cDNA开始，我们进行了比较和系统进化序列分析，研究了脊椎动物进化过程中该基因的保守性，并定义了斑马鱼发育过程中的组织表达。完整安装原位杂交首先检测到slc7a1a受精后14 h（hpf）在体，眼和脑中的转录本，在受精后24 hpf（dpf）在远端肾单位和在arch弓中进一步表达（dpf），显着增加5 dpf。综上所述，斑马鱼Cat-1系统基因slc7a1a的表达分析表明，在中枢神经系统，肌肉，腮和肾脏的早期发育过程中发挥了一定的功能。