Increasing demands for bioactive compounds have motivated researchers to employ micro‐organisms to produce complex natural products. Currently, Bacillus subtilis has been attracting lots of attention to be developed into terpenoids cell factories due to its generally recognized safe status and high isoprene precursor biosynthesis capacity by endogenous methylerythritol phosphate (MEP) pathway. In this review, we describe the up‐to‐date knowledge of each enzyme in MEP pathway and the subsequent steps of isomerization and condensation of C5 isoprene precursors. In addition, several representative terpene synthases expressed in B. subtilis and the engineering steps to improve corresponding terpenoids production are systematically discussed. Furthermore, the current available genetic tools are mentioned as along with promising strategies to improve terpenoids in B. subtilis, hoping to inspire future directions in metabolic engineering of B. subtilis for further terpenoid cell factory development.

中文翻译：

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将枯草芽孢杆菌定位为类萜细胞工厂

对生物活性化合物的需求不断增长，促使研究人员采用微生物来生产复杂的天然产物。目前，枯草芽孢杆菌由于其普遍公认的安全状态和通过内源性甲基赤藓糖醇磷酸（MEP）途径的高异戊二烯前体生物合成能力，已引起人们的广泛关注，以开发为类萜细胞工厂。在这篇综述中，我们描述了MEP途径中每种酶的最新知识以及C5异戊二烯前体的异构化和缩合的后续步骤。此外，在枯草芽孢杆菌中表达了几种代表性的萜烯合酶并系统地讨论了改善相应萜类化合物生产的工程步骤。此外，当前可用的遗传工具被提及作为有希望的战略，以提高萜类沿枯草芽孢杆菌，希望能够激励中的代谢工程的未来方向枯草芽孢杆菌进一步萜类化合物的细胞工厂的发展。