Solar radiation exposure is recognised to be a significant contributor to the development of skin cancer. Monitoring the simultaneous and consecutive mechanisms of interaction could provide a greater understanding of the process of photocarcinogenesis. This work presents an analysis of the biochemical and morphological changes occurring to immortalised human epithelial keratinocyte (HaCaT) cell cultures exposed to simulated solar radiation (SSR). Cell viability was monitored with the aid of the Alamar Blue assay, morphological examination was done with haematoxylin and eosin staining (H&E) and changes to the biochemical constituents (nucleic acids and proteins) as a result of the radiation insult were demonstrated through a combination of Raman microspectroscopy and multivariate analysis of spectral patterns. The spectral results suggest that SSR induces changes to the conformational structure of DNA as an immediate result of the radiation, whereas alteration in the protein signature is mostly seen as a later response.

中文翻译：

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通过拉曼光谱法监测暴露于太阳辐射的人角质形成细胞发生的生化变化

公认太阳辐射暴露是皮肤癌发展的重要因素。监测相互作用的同时发生和连续发生的机制可以提供对光致癌过程的更多了解。这项工作提供了对暴露于模拟太阳辐射（SSR）的永生化人类上皮角质形成细胞（HaCaT）细胞培养物发生的生化和形态变化的分析。借助Alamar Blue分析法监测细胞活力，用苏木精和曙红染色（H＆E）进行形态学检查，并通过结合使用证明了辐射损伤后生化成分（核酸和蛋白质）的变化拉曼光谱和光谱图的多元分析。