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Overexpression of the homoterpene synthase gene, OsCYP92C21, increases emissions of volatiles mediating tritrophic interactions in rice
Plant, Cell & Environment ( IF 7.3 ) Pub Date : 2020-10-25 , DOI: 10.1111/pce.13924
Wei Li 1 , Lingnan Wang 1 , Fei Zhou 1 , Changyan Li 1 , Weihua Ma 1, 2 , Hao Chen 1 , Guirong Wang 3 , John A Pickett 4 , Jing-Jiang Zhou 5, 6 , Yongjun Lin 1
Affiliation  

Plant defence homoterpenes can be used to attract pest natural enemies. However, the biosynthetic pathway of homoterpenes is still unknown in rice, and the practical application of such indirect defence systems suffers from inherent limitations due to their low emissions from plants. Here, we demonstrated that the protein OsCYP92C21 is responsible for homoterpene biosynthesis in rice. We also revealed that the ability of rice to produce homoterpenes is dependent on the subcellular precursor pools. By increasing the precursor pools through specifically subcellular targeting expression, genetic transformation and genetic introgression, we significantly enhanced homoterpene biosynthesis in rice. The final introgressed GM rice plants exhibited higher homoterpene emissions than the wild type rice and the highest homoterpene emission reported so far for such GM plants even without the induction of herbivore attack. As a result, these GM rice plants demonstrated strong attractiveness to the parasitic wasp Cotesia chilonis. This study discovered the homoterpene biosynthesis pathway in rice, and lays the foundation for the utilisation of plant indirect defence mechanism in the “push‐pull” strategy of integrated pest management through increasing precursor pools in the subcellular compartments and overexpressing homoterpene synthase by genetic transformation.

中文翻译:

高萜类合酶基因OsCYP92C21的过表达增加了介导水稻三养相互作用的挥发物排放

植物防御高萜类化合物可用于吸引害虫天敌。然而,高萜类化合物的生物合成途径在水稻中仍然是未知的,并且这种间接防御系统的实际应用由于其从植物中的低排放而受到固有的限制。在这里,我们证明了蛋白质OsCYP92C21负责水稻中的同萜生物合成。我们还揭示了水稻产生高萜的能力取决于亚细胞前体库。通过特别通过亚细胞靶向表达,遗传转化和基因渗入增加前体库,我们显着增强了水稻中的同萜生物合成。最终渗入基因改造的水稻植物显示出高于野生型水稻的高三萜排放,即使没有诱导食草动物侵袭,迄今为止报道的此类转基因植物最高的三萜排放。结果,这些转基因水稻植物显示出对寄生黄蜂的强吸引力。Cotesia chilonis。这项研究发现了水稻中的同萜生物合成途径,并通过增加亚细胞区室中的前体库和通过遗传转化过表达同萜合酶,为植物间接防御机制在病虫害综合治理的“推挽”策略中的利用奠定了基础。
更新日期:2020-10-25
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