Polyreactive antibodies (pAb) bind to a broad range of unrelated structures, providing hosts with functional components able to rapidly recognize and protect against different pathogens. However, their roles against helminth parasites are still unexplored. Here, pAb profiles were analysed in cystic echinococcosis (CE), a zoonosis caused by the cestode Echinococcus granulosus sensu lato. Levels of anti‐DNP (2,4‐dinitrophenyl‐hapten) antibodies were measured as a surrogate parameter of pAb in different biological settings. Firstly, levels of serum and peritoneal pAb were measured during early experimental secondary CE, using both high (Balb/c) and low (C57Bl/6) susceptible mouse strains. Serum pAb mostly differed in normal mice, being pAb levels of IgG subclasses with poor anti‐parasite activities predominant in Balb/c animals. Conversely, peritoneal pAb isotypes/subclasses with efficient anti‐parasite activities predominated in normal and infected C57Bl/6 mice. Secondly, sera from potentially resistant patients, susceptible individuals and healthy donors were analysed, showing higher pAb levels of the IgA and IgG—particularly IgG1—isotypes in potentially resistant individuals compared to control groups. Finally, since remarkable differences were observed in pAb profiles according to the intrinsic host susceptibility to the infection, we proposed here that pAb might be considered as potential humoral biomarkers for host resistance to CE.

中文翻译：

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多反应性抗体可作为宿主对囊性棘球co虫病抗性的潜在体液生物标志物

多反应性抗体（pAb）结合多种不相关的结构，为宿主提供能够快速识别并防御不同病原体的功能成分。但是，它们对蠕虫寄生虫的作用仍未探索。在这里，分析了在囊性棘球co虫病（CE）中的pAb谱图，这是由尾est棘球chin虫引起的人畜共患病。在不同的生物环境中，抗-DNP（2,4-二硝基苯基-半抗原）抗体的水平作为pAb的替代参数进行了测量。首先，在早期实验性继发性CE中使用高（Balb / c）和低（C57Bl / 6）易感小鼠品系测量血清和腹膜pAb的水平。血清中的pAb在正常小鼠中主要不同，这是Balb / c动物中主要具有抗寄生虫活性差的IgG亚类的pAb水平。相反，在正常和感染的C57Bl / 6小鼠中，具有有效抗寄生虫活性的腹膜pAb亚型/亚类占主导地位。其次，分析了来自潜在耐药患者，易感人群和健康供体的血清，与对照组相比，显示出潜在耐药人群中IgA和IgG（尤其是IgG1）同种型的pAb水平更高。最后，