At this time, green analytical chemistry is gaining more interest and concern. The present work details three green spectrofluorimetric methods for tramadol (TRM) determination using ibuprofen (IBU) (mixture 1) and chlorzoxazone (CLZ) (mixture 2). In first method, two excitation wavelengths (λ_ex), 220 and 280 nm, were used to record the emission spectra for IBU and TRM, respectively (mixture 1) followed by a first derivative treatment. For mixture 2, one λ_ex (280 nm) was optimum for both drugs followed by a first derivative technique for TRM and a second derivative for CLZ determinations. The second method measured the first derivative values for synchronous spectra using constant‐wavelength mode at 280 nm for TRM and 260 nm for IBU, and at 270 nm for TRM and 292 nm for CLZ. The third method used constant‐energy mode to record synchronous spectra. First derivative values were computed at 282 nm for TRM and 260 nm for IBU in mixture 1 and at 272 nm for TRM and 292 nm for CLZ in mixture 2. ICH validation guidelines were assessed in full and assay of the two TRM binary mixtures in their drug products was successful. Green profile evaluation for the developed methods compared with the reported chromatographic methods was performed using the ‘analytical eco‐scale’ and the ‘green analytical procedure index’. These two assessment tools corroborated that the proposed methods achieved the most green parameters, therefore recommending their use as a green option for analyzing the studied drugs in bulk and dosage forms for routine quality control.

中文翻译：

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布洛芬或氯唑沙宗用于曲马多测定的绿色荧光光谱法：绿色曲线的比较

这时，绿色分析化学越来越引起人们的关注和关注。本工作详细介绍了使用布洛芬（IBU）（混合物1）和氯唑沙宗（CLZ）（混合物2）测定曲马多（TRM）的三种绿色荧光光谱法。在第一种方法中，分别使用220和280 nm的两个激发波长（_λex）记录IBU和TRM的发射光谱（混合物1），然后进行一阶导数处理。对于混合物2，一个_λex（280 nm）对于两种药物都是最佳选择，其后是TRM的一阶导数技术和CLZ测定的二阶导数。第二种方法使用恒定波长模式在TRM的280 nm和IBU的260 nm，TRM的270 nm和CLZ的292 nm处测量了同步光谱的一阶导数值。第三种方法使用恒定能量模式来记录同步光谱。在混合物1中，TRM在282 nm处计算IBU的一阶导数值，在混合物2中对于IBU在260 nm处计算的CLM，对于CLZ在292 nm处计算的一阶导数。在ICH验证指南中进行了全面评估，并分析了两种TRM二元混合物在其中的含量。药品成功。使用“分析型生态规模”和“绿色分析程序指数”，对已开发方法与所报告的色谱方法进行了绿色轮廓评估。这两种评估工具证实了所提出的方法达到了最绿色的参数，因此建议将它们用作分析所研究药物的大包装和剂型以进行常规质量控制的绿色选择。