The genetic diversity of the BoLA‐DRB3 gene has been reported in different cattle breeds owing to its central role in the immune response. However, it is still unknown in hundreds of cattle breeds, especially native populations. Here, we studied BoLA‐DRB3 genetic diversity in Highland Creole cattle (CrAl) from Western Bolivia, raised at altitudes between 3800 and 4200 m. DNAs from 48 CrAl cattle were genotyped for BoLA‐DRB3 exon 2 alleles using polymerase chain reaction‐sequence‐based typing (PCR‐SBT). The results were compared with 1341 previously reported data from Tropical Creole cattle and other breeds raised in the region. Twenty‐three BoLA‐DRB3 alleles were identified in CrAl, including the BoLA‐DRB3*029:02 variant previously detected in other Creole cattle. Observed and expected heterozygosity were 0.87 and 0.93, respectively. Nucleotide diversity and the number of pairwise difference values were 0.078 and 19.46, respectively. The average number of nonsynonymous and synonymous substitutions were 0.037 and 0.097 for the entire BoLA‐DRB3 exon 2, and 0.129 and 0.388 for the antigen‐binding site, respectively. Venn analysis and the review of the IPD‐MHC database and the literature showed that 2 of 64 alleles were only detected in CrAl, including BoLA‐DRB3*029:01 previously reported in African cattle and *048:01 detected in Philippine cattle. Two additional alleles, BoLA‐DRB3*007:02 and *029:02, were only present in CrAl and Lowland Creole cattle. Principal Component Analysis (PCA) showed that Bolivian Creole cattle breeds were closely located but they were distant from the Colombian Hartón del Valle Creole. F_ST analysis showed a low degree of genetic differentiation between Highland and Lowland Bolivian Creole cattle (F_ST = 0.015). The present results contribute to increasing our knowledge of BoLA‐DRB3 genetic diversity in cattle breeds.

中文翻译：

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玻利维亚高地克里奥尔人牛的BoLA‐DRB3遗传多样性

由于BoLA‐DRB3基因在免疫应答中起着重要作用，因此已在不同的牛品种中报告了其遗传多样性。但是，在数百个牛品种中，尤其是在本地种群中，它仍然是未知的。在这里，我们研究了玻利维亚西部高地克里奥尔人牛（CrAl）的BoLA‐DRB3遗传多样性，该牛在3800至4200 m的海拔高度饲养。使用聚合酶链反应基于序列的分型（PCR-SBT）对来自48名CrAl牛的DNA进行BoLA‐DRB3外显子2等位基因的基因分型。将结果与以前报告的1341种热带克里奥尔人牛和该地区其他品种的数据进行了比较。在CrAl中鉴定出23个BoLA-DRB3等位基因，包括BoLA-DRB3 * 029：02以前在其他克里奥尔人的牛中检测到的变种。观察到的杂合度和预期的杂合度分别为0.87和0.93。核苷酸多样性和成对差异值的数目分别为0.078和19.46。整个BoLA-DRB3外显子2的非同义和同义取代的平均数分别为0.037和0.097，抗原结合位点的分别为0.129和0.388。Venn分析以及对IPD-MHC数据库和文献的审查显示，仅在CrAl中检测到64个等位基因中的2个，包括先前在非洲牛中报告的BoLA-DRB3 * 029：01和在菲律宾牛中检测到的* 048：01。另外两个等位基因BoLA‐DRB3 * 007：02和* 029：02，仅存在于CrAl和低地克里奥尔人的牛中。主成分分析（PCA）表明，玻利维亚克里奥尔人的牛品种位于附近，但与哥伦比亚哈顿·德尔瓦莱克里奥尔人相去甚远。F _ST分析显示高地和低地玻利维亚克里奥尔人之间的遗传分化程度较低（F _ST = 0.015）。目前的结果有助于增加我们对牛品种中BoLA‐DRB3遗传多样性的了解。