Dendritic cells (DCs) play a crucial role in the complex interplay between tumor cells and the immune system. During the elimination phase of cancer immunoediting, immunostimulatory DCs are critical for the control of tumor growth. During the escape phase, regulatory DCs sustain tumor tolerance and contribute to the development of the immunosuppressive tumor microenvironment that characterizes this phase. Moreover, increasing evidence indicates that DCs are also critical for the success of cancer immunotherapy. Hence, there is increasing need to fully characterize DC subsets and their activatory/inhibitory profile in cancer patients. In this review, we describe the role played by different DC subsets in the different phases of cancer immunoediting, the function exerted by different activatory and inhibitory molecules expressed on DC surface, and the cytokines produced by distinct DC subsets, in order to provide an overview on the DC features that may be useful to be assessed when dealing with the flow cytometric characterization of DCs in cancer patients. © 2020 International Society for Advancement of Cytometry

中文翻译：

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流式细胞术对癌症患者树突状细胞的综合表型分析

树突状细胞 (DC) 在肿瘤细胞和免疫系统之间的复杂相互作用中起着至关重要的作用。在癌症免疫编辑的消除阶段，免疫刺激性 DCs 对于控制肿瘤生长至关重要。在逃逸阶段，调节性 DC 维持肿瘤耐受性，并有助于形成该阶段特征的免疫抑制性肿瘤微环境。此外，越来越多的证据表明 DC 对于癌症免疫治疗的成功也至关重要。因此，越来越需要充分表征癌症患者的 DC 亚群及其激活/抑制特征。在这篇综述中，我们描述了不同 DC 亚群在癌症免疫编辑的不同阶段所发挥的作用，DC 表面表达的不同激活和抑制分子所发挥的功能，以及由不同 DC 子集产生的细胞因子，以提供有关 DC 特征的概述，这些特征可能有助于在处理癌症患者 DC 的流式细胞术表征时进行评估。© 2020 国际细胞计量学促进会