T lymphocytes play a central role in antigen‐specific immune responses. They modulate the function of different immune cells both through a direct contact (receptor binding) and through the secretion of cytokines. At the same time, they are deeply involved in the direct killing of aberrant target cells. T lymphocytes derive from a bone marrow precursor that migrates in the thymus where the main differentiation steps take place. Mature CD4 and CD8 single‐positive cells, then, leave the thymus to reach the secondary lymphoid organs. T‐cell subsets and their maturation steps can be identified mainly based on the expression of extracellular markers, intracellular transcription factors and cytokine production profiles. In this review, we report, from a cytometric point of view, an overview of the most important T‐cell subpopulations and their differentiation state. © 2020 International Society for Advancement of Cytometry

中文翻译：

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健康小鼠外周血 T 淋巴细胞的综合表型分析

T 淋巴细胞在抗原特异性免疫反应中发挥核心作用。它们通过直接接触（受体结合）和细胞因子的分泌来调节不同免疫细胞的功能。同时，它们深入参与了异常靶细胞的直接杀伤。T 淋巴细胞源自骨髓前体，其在主要分化步骤发生的胸腺中迁移。然后，成熟的 CD4 和 CD8 单阳性细胞离开胸腺到达次级淋巴器官。T细胞亚群及其成熟步骤可以主要基于细胞外标志物、细胞内转录因子和细胞因子产生谱的表达来识别。在这篇综述中，我们从细胞计数的角度报告，最重要的 T 细胞亚群及其分化状态的概述。© 2020 国际细胞计量学促进会