There is a rising interest in viral and non‐viral gene delivery vehicles for human gene therapy and vaccination applications, and these vehicles include nanoparticulate virus, virus‐like particles (VLP) and plasmids. This communication debates the importance of designing innovative platforms for virus and VLP concentration and purification, envisaging downstream process integration and intensification. Two alternative separation platforms are suggested and discussed: affinity‐aqueous two‐phase systems and monolithic chromatography. These can integrate clarification, concentration, and purification downstream unit operations, significantly decreasing industrial manufacturing costs. Additionally, high recovery yields are expected after process optimization,which is achieved through manipulation of the physicochemical parameters governing partition coefficients. Such cutting‐edge technologies encompass several advantages at industrial scale, particularly in continuous operations, for example ease of scalability, continuous operation potential, easy process integration, low toxicity, and high biocompatibility. Together with innovative processing conditions, they can effectively overcome the major bottlenecks of conventional virus and VLP downstream processing, contributing to more efficient, sustainable, and cost effective industrial systems. © 2020 Society of Chemical Industry (SCI)

中文翻译：

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水两相系统和整体色谱作为病毒和类病毒颗粒纯化的替代技术平台

对用于人类基因治疗和疫苗接种的病毒和非病毒基因递送载体的兴趣日益浓厚，这些载体包括纳米微粒病毒，病毒样颗粒（VLP）和质粒。该交流讨论了设计创新平台以进行病毒和VLP浓缩和纯化的重要性，并设想了下游过程的整合和强化。建议并讨论了两种替代的分离平台：亲和水两相系统和整体色谱。这些可以整合澄清，浓缩和纯化下游单元操作，从而大大降低工业制造成本。此外，优化工艺后有望获得高回收率，这是通过控制支配系数的理化参数来实现的。这种尖端技术在工业规模上具有多个优势，特别是在连续操作中，例如易于扩展，连续操作潜力，易于工艺集成，低毒性和高生物相容性。结合创新的加工条件，它们可以有效克服常规病毒和VLP下游加工的主要瓶颈，从而有助于建立更高效，可持续和成本有效的工业系统。©2020年化学工业协会（SCI）低毒，高生物相容性。结合创新的加工条件，它们可以有效克服常规病毒和VLP下游加工的主要瓶颈，从而有助于建立更高效，可持续和成本有效的工业系统。©2020年化学工业协会（SCI）低毒，高生物相容性。结合创新的加工条件，它们可以有效克服常规病毒和VLP下游加工的主要瓶颈，从而有助于建立更高效，可持续和成本有效的工业系统。©2020年化学工业协会（SCI）