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Micro‐morpho‐anatomical mechanisms involve in epiphytic adaptation of micropropagated plants of Vanda tessellata (Roxb.) Hook. ex G. Don
Microscopy Research and Technique ( IF 2.5 ) Pub Date : 2020-10-22 , DOI: 10.1002/jemt.23630
Manokari Mani 1, 2 , Latha Rasangam 2 , Priyadharshini Selvam 2 , Mahipal Singh Shekhawat 2
Affiliation  

Vanda tessellata (Roxb.) Hook. ex G. Don. (grey orchid, family Orchidaceae) is an epiphytic orchid of horticultural importance and currently under threat due to overharvesting and habitat destruction. Micropropagation protocols were developed for the production of grey orchid but the survival success of in vitro regenerated plantlets is uncertain due to lack of understanding about the adaptation mechanism during hardening. The present study describes the structural adaptation mechanism of V. tessellata when the in vitro regenerated plantlets were acclimatized under the greenhouse conditions. Light microscopy has been implicated to identify the adaptational alterations during in vitro to ex vitro transition of micropropagated plantlets. The in vitro induced morpho‐anatomical anomalies were more prominently observed in the density of stomata, veins (architecture) and raphides, leaf, and root structural parameters such as water cells and velamen tissues. The results indicated that remarkable reconciliation occurred in structural developments of mechanical and vascular tissues upon epiphytic adaptations of V. tessellata. The study could help in understanding the adaptation mechanism of in vitro regenerated plantlets (especially velamen tissues of epiphytic roots) when transferred to the greenhouse for acclimatization.

中文翻译:

微形态解剖机制涉及 Vanda tesselata (Roxb.) Hook 微繁殖植物的附生适应。前 G. 唐

Vanda tessellata (Roxb.) 钩。前 G. 唐。(灰兰花,兰花科)是一种具有园艺重要性的附生兰花,目前由于过度采伐和栖息地破坏而受到威胁。微繁殖方案是为生产灰兰花而开发的,但由于缺乏对硬化过程中的适应机制的了解,体外再生植株的存活成功率尚不确定。本研究描述了V. tesselata的结构适应机制当体外再生的幼苗在温室条件下驯化时。光学显微镜已被用于鉴定微繁殖小植株的体外到体外转变过程中的适应性改变。体外诱导的形态解剖异常在气孔、静脉(结构)和针叶、叶和根结构参数(如水细胞和维拉门组织)的密度中观察到的更为显着。结果表明,在V. tesselata 的附生适应过程中,机械和维管组织的结构发育发生了显着的协调. 该研究有助于了解体外再生植株(特别是附生根的维拉门组织)转移到温室进行驯化时的适应机制。
更新日期:2020-10-22
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