Breast cancer is the most prevalent cancer in women. X‐linked inhibitor of apoptosis protein (XIAP) that is constantly overexpressed in cancer is a promising therapeutic target in cancer treatments. The mechanisms of the anticancer effects of carotenoid isolates of Spondias mombim in DMBA‐induced breast cancer in Wistar rats through XIAP antagonism were investigated in the present study. Carotenoids isolated from the leaves of Spondias mombim were subjected to Liquid Chromatography/Mass Spectrometry (LC/MS) and Electrospray Ionization (ESI) for characterization. The characterized carotenoid isolates were docked against XIAP BIR2 domain and XIAP BIR3 domain. The anticancer effects of the carotenoid isolates of Spondias mombim in DMBA‐induced breast cancer in Wistar rats were also investigated through the expression of XIAP, COX‐2, TNF, BCl‐2 mRNAs by qRT‐PCR and biochemical parameters of catalase, lipid peroxidation, LDH, ALP, and ALT. These show the carotenoid isolates demonstrate anticancer effects by antagonism of XIAP, proapoptotic, and anti‐inflammatory properties.

中文翻译：

---

通过对X连锁的凋亡蛋白抑制剂（XIAP）的拮抗作用和抗炎作用，Spondias mombin的类胡萝卜素分离物对DMBA诱导的Wistar大鼠乳腺癌具有抗癌作用。

乳腺癌是女性中最普遍的癌症。在癌症中持续过度表达的X连锁凋亡蛋白（XIAP）抑制剂是癌症治疗中有希望的治疗靶标。通过XIAP拮抗作用，研究了Spondias mombim类胡萝卜素分离物对Dstar诱导的Wistar大鼠乳腺癌的抗癌作用机理。从苦瓜的叶子中分离出的类胡萝卜素经过液相色谱/质谱（LC / MS）和电喷雾电离（ESI）进行表征。将表征的类胡萝卜素分离物对接XIAP BIR2结构域和XIAP BIR3结构域。Spondias mombim类胡萝卜素分离物的抗癌作用还通过qRT-PCR通过过氧化氢酶（XIAP），COX-2，TNF，BCl-2 mRNA的表达以及过氧化氢酶，脂质过氧化，LDH，ALP和ALT的生化参数研究了Wistar大鼠在DMBA诱导的乳腺癌中的表达。这些表明类胡萝卜素分离物通过XIAP的拮抗作用，促凋亡和抗炎特性表现出抗癌作用。