In this study, the influence of in vitro gastrointestinal digestion on the Perilla frutescens leaf extract (PFLE) were measured. Results revealed that total phenolic content (TPC) and total flavonoid content (TFC) were significantly decreased after simulated digestion (ca. 53% of phenolics and 40% of flavonoids). The IC₅₀ value of DPPH· scavenging activity and ABTS⁺ scavenging ability increased by 23% and 56%, respectively, while ferric reducing antioxidant power reduced by 53%. For the inhibition ability on α‐glucosidase, acetylcholinesterase, and MCF‐7 cell proliferation, their IC₅₀ values increased by 360%, 197%, and 25%, respectively. Three phenolic acids and one flavonoid in PFLE were quantified by high‐performance liquid chromatography. Overall, although significant losses of the active components and biological activities occurred during in vitro gastrointestinal digestion, it still showed the potential as an oral agent for treatment and prevention of oxidative stress, cancer, diabetes, and Alzheimer's disease.

中文翻译：

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体外胃肠道消化对紫苏叶提取物的影响：活性成分和生物活性的变化

在这项研究中，测量了体外消化对紫苏叶提取物（PFLE）的影响。结果显示，模拟消化后总酚含量（TPC）和总黄酮含量（TFC）显着降低（约53％的酚类和40％的黄酮类）。DPPH·清除活性和ABTS ⁺清除能力的IC ₅₀值分别提高了23％和56％，而三氧化二铁的抗氧化能力降低了53％。对于α-葡萄糖苷酶，乙酰胆碱酯酶和MCF-7细胞增殖的抑制能力，其IC ₅₀值分别增加了360％，197％和25％。高效液相色谱法定量分析了PFLE中的三种酚酸和一种类黄酮。总体而言，尽管在体外胃肠消化过程中活性成分和生物活性显着降低，但它仍显示出作为口服剂治疗和预防氧化应激，癌症，糖尿病和阿尔茨海默氏病的潜力。